Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Autor: Paul Honegger, Gerhard Gstraunthaler, Å. Fex Svenningsen, Toni Lindl, J.B.F. van der Valk, D. Brunner, Lisbeth E. Knudsen, M.L. Scarino, Jens Noraberg, K De Smet, Anna Price

Publikováno v: van der Valk, J, Brunner, D, De Smet, K, Fex Svenningsen, Å, Honegger, P, Knudsen, L E, Lindl, T, Noraberg, J, Price, A, Scarino, M L & Gstraunthaler, G 2010, ' Optimization of chemically defined cell culture media-Replacing fetal bovine serum in mammalian in vitro methods ', Toxicology in Vitro, vol. 24, no. 4, pp. 1053-1063 . https://doi.org/10.1016/j.tiv.2010.03.016

Udgivelsesdato: Mar-31 Quality assurance is becoming increasingly important. Good laboratory practice (GLP) and good manufacturing practice (GMP) are now established standards. The biomedical field aims at an increasing reliance on the use of in vitr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::196f0c312699dda6f1aedb833fbc1c44
https://doi.org/10.1016/j.tiv.2010.03.016

A novel in vitro metabolomics approach for neurotoxicity testing, proof of principle for methyl mercury chloride and caffeine

Autor: Siegfried Morath, Sandra Coecke, Jens P. Linge, Paul Honegger, Juri Rappsilber, Chantra Eskes, Thomas Hartung, Erwin van Vliet

Publikováno v: NeuroToxicology. 29:1-12

There is a need for more efficient methods giving insight into the complex mechanisms of neurotoxicity. Testing strategies including in vitro methods have been proposed to comply with this requirement. With the present study we aimed to develop a nov

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0f554a4cce80dd7101ece8e9d56e083
https://doi.org/10.1016/j.neuro.2007.09.007

Tumor necrosis factor-? and ?B-crystallin up-regulation during antibody-mediated demyelination in vitro: A putative protective mechanism in oligodendrocytes

Autor: Paul Honegger, Jean-Marie Matthieu, Florianne Monnet-Tschudi, Cécile Besson Duvanel, Olivier Braissant

Publikováno v: Journal of Neuroscience Research, vol. 78, no. 5, pp. 711-722

By using an in vitro model of antibody-mediated demyelination, we investigated the relationship between tumor necrosis factor-alpha (TNF-alpha) and heat shock protein (HSP) induction with respect to oligodendrocyte survival. Differentiated aggregate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03561986f346b4974effe6b490a7b8d4
https://doi.org/10.1002/jnr.20310

Alteration of amino acid metabolism in neuronal aggregate cultures exposed to hypoglycaemic conditions

Autor: Paul Honegger, Claude Bachmann, Olivier Braissant, Marie-Gabrielle Zurich, Olivier Boulat, Hugues Henry, Beatriz Pardo

Publikováno v: Journal of Neurochemistry. 81:1141-1151

The neuronal effects of glucose deficiency on amino acid metabolism was studied on three-dimensional cultures of rat telencephalon neurones. Transient (6 h) exposure of differentiated cultures to low glucose (0.25 mm instead of 25 mm) caused irrevers

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4f5f1c8a5ab5aaed1d6a96b1d89d7b9b
https://doi.org/10.1046/j.1471-4159.2002.00888.x

[Untitled]

Autor: Paul Honegger, S. Pouly, Jean-Marie Matthieu

Publikováno v: Neurochemical Research. 26:619-627

In previous work we found that mezerein, a C kinase activator, as well as basic fibroblast growth factor (FGF-2) induce demyelination and partial oligodendrocyte dedifferentiation in highly differentiated aggregating brain cell cultures. Here we show

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8a27eeb767d5bc3b85900afb6eab8798
https://doi.org/10.1023/a:1010931102287