Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Autor: Clara J. Douet, David L. Hacker, Stéphanie Tissot, Lucia Baldi, Florian M. Wurm, Patrik O. Michel, Sarah Grezet

Publikováno v: Processes
Volume 1
Issue 1
Pages 3-11
Processes, Vol 1, Iss 1, Pp 3-11 (2013)

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (kLa) was 200%–400% higher in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1f1f85c815bf82a132e87b65c91ff94
https://doi.org/10.3390/pr1010003

Purification and analysis of polyhistidine-tagged human parvovirus B19 VP1 and VP2 expressed in insect cells

Autor: Lea Hedman, Patrik O. Michel, Christian Oker-Blom, Jouni Toivola, Maria Söderlund-Venermo, Anna R. Mäkelä, Klaus Hedman, Eila Korhonen

Publikováno v: Journal of Virological Methods. 152:1-5

Human parvovirus B19 is an autonomously replicating human pathogen with a specific tropism for human erythroid progenitor cells. There is an interest in producing empty nucleocapsids of B19 as they can be used as tools in molecular biology and diagno

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbc3734220357b9463e46b0d53640391
https://doi.org/10.1016/j.jviromet.2008.06.006

Expression and purification of polyhistidine-tagged firefly luciferase in insect cells

Autor: Patrik O. Michel, Tuula K. Torkkeli, Christian Oker-Blom, Matti Karp

Publikováno v: Michel, P, Torkkeli, T, Karp, M & Oker-Blom, C 2001, ' Expression and purification of polyhistidine-tagged firefly luciferase in insect cells : A potential alternative for process scale-up ', Journal of Biotechnology, vol. 85, no. 1, pp. 49-56 . https://doi.org/10.1016/S0168-1656(00)00377-1

The coleopteran firefly, Photinus pyralis, luciferase was produced in lepidopteran Trichoplusia ni insect cells using a baculovirus expression vector. The recombinant protein was equipped with a polyhistidine affinity tag at the carboxyl terminus and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40e2721ef0e5c57601b36d3a226b7fab
https://cris.vtt.fi/en/publications/b81a9102-3143-4a7e-8c0c-aff986d3bd52

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Autor: Lucia Baldi, David L. Hacker, Xiaowei Zhang, Florian M. Wurm, Qiuling Xie, Patrik O. Michel

Publikováno v: Biotechnology letters. 33(5)

Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 10(6) cells/m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe99c58d9467e1f8a9758dd1d10f7b0a
https://pubmed.ncbi.nlm.nih.gov/21267765

Disassembly of structurally modified viral nanoparticles: characterization by fluorescence correlation spectroscopy

Autor: Matti Vuento, Daniel J. White, Jouni Toivola, Patrik O. Michel, Leona Gilbert, Christian Oker-Blom

Publikováno v: Comptes rendus biologies. 328(12)

Analysis of the breakdown products of engineered viral particles can give useful information on the particle structure. We used various methods to breakdown both a recombinant enveloped virus and virus-like particles (VLPs) from two non-enveloped vir

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::357511643b7e7212d6f04e552a9b14cd
https://pubmed.ncbi.nlm.nih.gov/16314283