Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Patrik Dahlen"'
Autor:
Veli-Matti Mukkala, Marek Kwiatkowski, Liisa Liukkonen, Jyrki Ylikoski, Antti Iitiä, Patrik Dahlen, Timo Lövgren, Harri Siitari, Pertti Hurskainen
Publikováno v:
Bioconjugate Chemistry. 5:268-272
A chemical method for labeling of oligonucleotide probes with europium chelates is presented. A modified deoxycytidine phosphoramidite is used to introduce multiple reactive amino groups to the oligonucleotide during the synthesis phase. Upon deprote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae1242ca6123d5529066c44c9bfc063a
https://doi.org/10.1021/bc00027a013
https://doi.org/10.1021/bc00027a013
Autor:
Patrik Dahlen, Eeva Nikoskelainen, Harri Siitari, Kirsi Huoponen, Pertti Aula, Marja-Liisa Savontaus, Antti Iitiä, Vesa Juvonen
Publikováno v:
Human Mutation. 3:29-36
We have applied time-resolved fluorometry (TRF) to construct a DNA hybridization assay for the diagnosis of Leber hereditary optic neuroretinopathy (LHON). A rapid and reliable detection of the most prevalent mitochondrial DNA (mtDNA) point mutation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e254e56bff575af47d04d983e2b7b37f
https://doi.org/10.1002/humu.1380030106
https://doi.org/10.1002/humu.1380030106
Autor:
Jens Vuust, Antti Litiä, Jørgen Arends, Estrid Høgdall, Bent Nørgaard-Pedersen, Patrik Dahlen
Publikováno v:
Screening. 2:1-11
A total of 1081 newborn screening cards were anonymously selected for cystic fibrosis (CF) screening by quantitation of immunoreactive trypsin (IRT, Delfia) and by ΔF508 mutational analyses using polymerase chain reaction followed by both gel retard
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5015cba0d58a2bf708144fb5187b309c
https://doi.org/10.1016/0925-6164(93)90012-8
https://doi.org/10.1016/0925-6164(93)90012-8
Publikováno v:
Molecular and Cellular Probes. 5:143-149
The polymerase chain reaction (PCR) has many potential applications in the field of DNA probe diagnostics. Here we describe a method that utilizes PCR and time-resolved fluorometry (TRF) for the detection of specific target DNA. First the DNA segment
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0754e20fdd0b2db70f5cbdf4def418bb
https://doi.org/10.1016/0890-8508(91)90009-9
https://doi.org/10.1016/0890-8508(91)90009-9
Autor:
Marek Kwiatkowski, Jyrki Ylikoski, Patrik Dahlen, Harri Siitari, Timo Lövgren, Pertti Hurskainen
Publikováno v:
Nucleic Acids Research. 19:1057-1061
A chemical method for labelling DNA with a europium chelate is presented. First, primary aliphatic amino groups are introduced onto DNA in a transamination reaction. The transamination reaction is altered by adjusting temperature and duration of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ac060045934a2948c605931a49c8868
https://doi.org/10.1093/nar/19.5.1057
https://doi.org/10.1093/nar/19.5.1057
Autor:
Patrik Dahlen, V. Näntö, Antti Iitiä, A. Rajamäki, Jarkko U. Eskola, Harri Siitari, Mauri M. Hämäläinen
Publikováno v:
Clinical biochemistry. 27(5)
More than 95% of the patients with chronic myelogenous leukemia (CML) carry translocations between protooncogene abl of chromosome 9 and bcr gene of chromosome 22, resulting in the Philadelphia chromosome (Ph1). After allogeneic bone marrow transplan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84ade26643797e4ebdcdce6bafb07d4b
https://pubmed.ncbi.nlm.nih.gov/7867215
https://pubmed.ncbi.nlm.nih.gov/7867215
Publikováno v:
Analytical biochemistry. 202(1)
Since its discovery, the polymerase chain reaction (PCR) has been used for different purposes in the field of DNA research. We tested the PCR for the diagnosis of HTLV-I/-II infections. PCR was used to amplify 141- and 149-base pair regions from the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2fe9960a85c2dafc3aaf36be5d0dc95
https://pubmed.ncbi.nlm.nih.gov/1621988
https://pubmed.ncbi.nlm.nih.gov/1621988
The polymerase chain reaction (PCR) has many potential applications in the field of nucleic acid diagnostics. In particular, it has been successfully applied to the detection of pathogens present in low copy numbers such as the human immunodeficiency
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87cfdf33667a308d50cdcc69f7296cbc
https://europepmc.org/articles/PMC269873/
https://europepmc.org/articles/PMC269873/
Autor:
Patrik Dahlen
Publikováno v:
Analytical Biochemistry. 164:78-83
Europium has been used as a label in immunoassays as it can be measured with high sensitivity by means of time-resolved fluorometry. Here we have used streptavidin labeled with europium chelates in the detection of adenovirus type 2 DNA bound to micr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3392305483df58cf0ae4da3fd8822947
https://doi.org/10.1016/0003-2697(87)90370-8
https://doi.org/10.1016/0003-2697(87)90370-8
Publikováno v:
Rapid Methods in Clinical Microbiology ISBN: 9781461278863
Radioactive isotopes are the most widely used markers in clinical chemistry. Although radioactive labels are very sensitive they have several drawbacks. The most severe disadvantages are the health hazards involved and the limited shelf life of reage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d26e9bf60736d7a984d8c0ec12510fa1
https://doi.org/10.1007/978-1-4613-0601-6_11
https://doi.org/10.1007/978-1-4613-0601-6_11