Decoding the folding of Burkholderia glumae lipase: folding intermediates en route to kinetic stability.

Autor: Kris Pauwels, Manuel M Sanchez del Pino, Georges Feller, Patrick Van Gelder

Publikováno v: PLoS ONE, Vol 7, Iss 5, p e36999 (2012)

The lipase produced by Burkholderia glumae folds spontaneously into an inactive near-native state and requires a periplasmic chaperone to reach its final active and secretion-competent fold. The B. glumae lipase-specific foldase (Lif) is classified a

Externí odkaz: https://doaj.org/article/76f7abacca374a66a2ac73486ac92f7c

The perspectives of older adults with mild cognitive impairment and their caregivers on the use of socially assistive robots in healthcare : exploring factors that influence attitude in a pre-implementation stage

Autor: Maaike Van Assche, Mirko Petrovic, Dirk Cambier, Patrick Calders, Patrick Van Gelder, Dominique Van de Velde

Publikováno v: DISABILITY AND REHABILITATION-ASSISTIVE TECHNOLOGY

Due to increasing age and an increasing prevalence rate of neurocognitive disorders such as Mild Cognitive Impairment (MCI) and dementia, independent living may become challenging. The use of socially assistive robots (SARs) is one solution that can

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6eaf4dcb887cd97711f5213a949c6b4f
https://hdl.handle.net/1854/LU-8753679

Hydrophobic surface patches on LoIA of Pseudomonas aeruginosa are essential for lipoprotein binding

Autor: Lieven Buts, Kim Remans, Peter van Ulsen, Kris Pauwels, Savvas N. Savvides, Patrick Van Gelder, Klaas Decanniere, Jan Tommassen, Pierre Cornelis

Publikováno v: Remans, K, Pauwels, K, van Ulsen, J P, Buts, L, Cornelis, P, Tommassen, J, Savvides, S N, Decanniere, K & van Gelder, P 2010, ' Hydrophobic surface patches on LoIA of Pseudomonas aeruginosa are essential for lipoprotein binding. ', Journal of Molecular Biology, vol. 401, no. 5, pp. 921-930 . https://doi.org/10.1016/j.jmb.2010.06.067
Journal of Molecular Biology, 401(5), 921-930. Academic Press Inc.

Many lipoproteins reside in the outer membrane (OM) of Gram-negative bacteria, and their biogenesis is dependent on the Lol (localization of lipoproteins) system. The periplasmic chaperone LolA accepts OM-destined lipoproteins that are released from

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9885878dd95676cd9a784b3d0ae5d36d
https://doi.org/10.1016/j.jmb.2010.06.067

Lipids revert inert Aβ amyloid fibrils to neurotoxic protofibrils that affect learning in mice

Autor: Patrick Van Gelder, Dieter Hartmann, Inna Kuperstein, Elke Maes, Frederic Rousseau, Wim Jonckheere, Mieke Vanbrabant, Joost Schymkowitz, Rudi D'Hooge, Hannah Wilkinson, Bart De Strooper, Ivo C. Martins

Publikováno v: The EMBO Journal

Although soluble oligomeric and protofibrillar assemblies of Abeta-amyloid peptide cause synaptotoxicity and potentially contribute to Alzheimer's disease (AD), the role of mature Abeta-fibrils in the amyloid plaques remains controversial. A widely h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14211f081b81301adc3baf32d5b10748
https://doi.org/10.1038/sj.emboj.7601953

Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate

Autor: Guy Vandenbussche, Jean Marie Ruysschaert, Patrick Van Gelder, Kris Pauwels, Remy Loris, Lode Wyns

Publikováno v: Acta Crystallographica Section F Structural Biology and Crystallization Communications. 61:791-795

Bacterial lipases that are secreted via the type II secretion pathway require a lipase-specific foldase in order to obtain their native and biologically active conformation in the periplasmic space. The lipase-foldase complex from Burkholderia glumae

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5ce9a0ed60f8bd83968f822396a3e5a
https://doi.org/10.1107/s1744309105023055