Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Patrick Stanssens"'
Publikováno v:
BioTechniques, Vol 32, Iss 6S, Pp S62-S69 (2002)
Externí odkaz:
https://doaj.org/article/489e5e863eaf447793094c46ada68031
Autor:
Saskia De Keyser, Peter Schotte, Isabelle Dewerte, Manu De Groeve, Veronique De Brabandere, Patrick Stanssens
Publikováno v:
Microbial Cell Factories
Background Over the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. Several P. pastoris engineered strains and mutants have been developed to
Autor:
Patrick Stanssens, Ronald van Eijk, Jan Willem F Dierssen, Hans Morreau, Anne-Marie Cleton-Jansen, Marjo van Puijenbroek, Tom van Wezel
Publikováno v:
The Journal of Molecular Diagnostics. 7:623-630
As the number of identified single-nucleotide polymorphisms (SNPs) increases, high-throughput methods are required to characterize the informative loci in large patient series. We investigated the feasibility of MassEXTEND LOH analysis using Sequenom
Autor:
Dirk van den Boom, Marc Zabeau, Charles P. Rodi, Christiane Honisch, Patrick Stanssens, Sebastian Böcker, Yannick Georges Jozef Gansemans, Gwen Remes, Niels Storm, Geert Meersseman, Ralf Hartmer
Publikováno v:
Genome Research. 14:126-133
We describe a comparative sequencing strategy that is based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyses of complete base-specific cleavage reactions of a target sequence. The target is conve
Publikováno v:
BioTechniques. 32:S62-S69
Autor:
H Depraetere, C. Wille, S De Reys, Yannick Georges Jozef Gansemans, Patrick Stanssens, Dominique Baruch, Hans Deckmyn, Marc Lauwereys
Publikováno v:
Thrombosis and Haemostasis. 77:0981-0985
SummaryThe integrin α2β1 is a major cellular receptor for collagen. The α2subunit contains an ± 200 amino acids inserted domain (I-domain) in the N-terminal region. A certain degree of homology exists between the I-domains found in integrins, col
Autor:
Y G Gansemans, Laurent S. Jespers, G P Vlasuk, Joris Messens, Patrick Stanssens, M Lauwereys, A De Keyser, I Van den Brande, Dominique Eeckhout
Publikováno v:
Nature Biotechnology. 13:378-382
We describe a novel phage display system that affords the surface expression and hence affinity selection of cDNAs. The strategy is based on a new approach to functionally display proteins on filamentous phage through the attachment to the C-terminus
Autor:
John K. Field, Charles R. Cantor, Matthew R. Nelson, Dirk van den Boom, Mathias Ehrich, Triantafillos Liloglou, George Xinarianos, Marc Zabeau, Patrick Stanssens
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 102(44)
Methylation is one of the major epigenetic processes pivotal to our understanding of carcinogenesis. It is now widely accepted that there is a relationship between DNA methylation, chromatin structure, and human malignancies. DNA methylation is poten
Autor:
Pieter Vos, Patrick Stanssens
Publikováno v:
Current Protocols in Molecular Biology
This unit presents an alternative to differential display that allows the quantification of transcripts, based on AFLP-fingerprinting of double-stranded cDNA. The protocol described includes the following steps: the isolation of poly(A)+ RNA from tot
Publikováno v:
Gene. 173(2)
We describe a vector, lambdaZLG6, combining the high efficiency of cDNA library cloning in bacteriophage lambda with filamentous phage display of cDNA-encoded products. The cDNAs are expressed as fusions to the 3' end of M13 gene VI. The lambdaZLG li