Výsledky vyhledávání - "Patrick Seguin"

Measurement of serum osteocalcin with a human-specific two-site immunoradiometric assay

Autor: Marc Grimaux, Corinne Preaudat, Patrick Seguin, Pierre D. Delmas, B. Demiaux, Patrick Garnero

Publikováno v: Journal of Bone and Mineral Research. 7:1389-1398

We developed a sensitive and specific two-site radioimmunoassay (IRMA) for human osteocalcin using human osteocalcin as a standard and two monoclonal antibodies raised against human osteocalcin purified from human cortical bone, a solid-phase anti-25

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d07750548bb9e3b7717b8cc6d0fce345
https://doi.org/10.1002/jbmr.5650071206

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Homogeneous Time Resolved Fluorescence Assay to Measure Histamine Release

Autor: Emmanuel J. Claret, Patrick Seguin, Josy Ouled-Diaf

Publikováno v: Combinatorial Chemistry & High Throughput Screening. 6:789-794

Histamine is a biogenic amine synthesized by the enzymatic decarboxylation of histidine. Implication of histamine in allergy is well described but histamine is also found in some specific neurones, functions as a neurotransmitter and regulates sleep/

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad7aeece98629c7be5294a9f605948b5
https://doi.org/10.2174/138620703771826892

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High-throughput miniaturized immunoassay for human interleukin-13 secreted from NK3.3 cells using homogenous time-resolved fluorescence

Autor: Koji Enomoto, Akiko Araki, Nakajima Toshihiro, Goro Kominami, Ryuji Suzuki, Marc Preaudat, Hiroshi Takemoto, Patrick Seguin, Keiji Dohi, Gérard Mathis, Hideki Ohta

Publikováno v: Journal of Pharmaceutical and Biomedical Analysis. 28:73-79

A miniaturized immunoassay for human interleukin-13 (IL-13) using homogeneous time-resolved fluorescence (HTRF®) has been developed. In this assay, IL-13 which was secreted from NK3.3 cells stimulated with interleukin-2 (IL-2) was detected by measur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcf513e39049cfe9866ed00ae91cc017
https://doi.org/10.1016/s0731-7085(01)00596-9

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Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin

Autor: Caroline Ligny-Lemaire, Corinne Chanussot, Jean-Claude Boulain, Laurent Bellanger, Patrick Seguin, André Ménez

Publikováno v: Journal of Immunological Methods. 197:39-49

A synthetic DNA encoding human proinsulin was inserted in frame in the bacterial alkaline phosphatase gene. A homogeneous recombinant human proinsulin-alkaline phosphatase conjugate was obtained directly from the periplasm of Escherichia coli transfo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81c8c9a607617341f7422f79e5f6fcd8
https://doi.org/10.1016/0022-1759(96)00109-3

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Characterization of immunoreactive forms of human osteocalcin generated in vivo and in vitro

Autor: Marc Grimaux, Pierre D. Delmas, Patrick Seguin, Patrick Garnero

Publikováno v: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 9(2)

Three monoclonal antibodies recognizing the 5-13, 25-37, and 43-49 sequence of the human osteocalcin were used in competitive and two-site radioimmunoassays (RIA) to characterize specifically various immunoreactive forms of circulating human osteocal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02ca04c675cea350c19127228e920300
https://pubmed.ncbi.nlm.nih.gov/8140939

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