Zobrazeno 1 - 2
of 2
pro vyhledávání: '"Patrick Priem"'
Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose
Autor:
Joseph Brown, Jackie J. Eckblad, Patrick Priem, Jolanda Gerritsen, Michael Gramer, Carrie Shultz, Kent Vickerman, Ewald T. J. van den Bremer, Patrick Van Berkel, Ruth Donahue
Publikováno v:
Biotechnology and Bioengineering. 108:1591-1602
Through process transfer and optimization for increased antibody production to 3 g/L for a GS-CHO cell line, an undesirable drop in antibody Fc galactosylation was observed. Uridine (U), manganese chloride (M), and galactose (G), constituents involve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::843196d8fe94ca2cf92b9a05ec7df625
https://doi.org/10.1002/bit.23075
https://doi.org/10.1002/bit.23075
Autor:
Ewald T. J. van den Bremer, Rob N. de Jong, Janine Schuurman, Arnout F. Gerritsen, Paul W. H. I. Parren, Joyce I. Meesters, Aran F. Labrijn, Muriel D. van Kampen, Patrick Priem
Publikováno v:
Nature protocols. 9(10)
Labrijn et al. describe the generation of bispecific antibodies through controlled Fab-arm exchange, involving separate expression of two parental IgG1s containing single matching point mutations, followed by recombination of the 'half molecules'. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5af1dee95da29ec0c894511a4a1866f0
https://pubmed.ncbi.nlm.nih.gov/25255089
https://pubmed.ncbi.nlm.nih.gov/25255089