Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Patrick Ovando-Roche"'
Autor:
Patrick Ovando-Roche, Emma L. West, Matthew J. Branch, Robert D. Sampson, Milan Fernando, Peter Munro, Anastasios Georgiadis, Matteo Rizzi, Magdalena Kloc, Arifa Naeem, Joana Ribeiro, Alexander J. Smith, Anai Gonzalez-Cordero, Robin R. Ali
Publikováno v:
Stem Cell Research & Therapy, Vol 9, Iss 1, Pp 1-14 (2018)
Abstract Background The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies and disease modelling relies on the ability to obtain healthy and organised retinal tissue in sufficient quantities. Generating such tissue i
Externí odkaz:
https://doaj.org/article/8cabbf931a364048830c83f3d92ee7ad
Autor:
Wei Cui, Patrick Ovando-Roche
Publikováno v:
Bio-Protocol, Vol 5, Iss 13 (2015)
Ubiquitination is the first step of the ubiquitin-proteasome pathway that regulates cells for their homeostatic functions and is an enzymatic, protein post-translational modification process in which ubiquitin is transferred to a target protein subst
Externí odkaz:
https://doaj.org/article/397a56a9b4b14e9aabd21391e9f50e4b
Autor:
Emma L. West, Paromita Majumder, Arifa Naeem, Milan Fernando, Michelle O'Hara-Wright, Emily Lanning, Magdalena Kloc, Joana Ribeiro, Patrick Ovando-Roche, Ian O. Shum, Neeraj Jumbu, Robert Sampson, Matt Hayes, James W.B. Bainbridge, Anastasios Georgiadis, Alexander J. Smith, Anai Gonzalez-Cordero, Robin R. Ali
Publikováno v:
Stem Cell Reports. 17:775-788
The generation of retinal organoids from human pluripotent stem cells (hPSC) is now a well-established process that in part recapitulates retinal development. However, hPSC-derived photoreceptors that exhibit well-organized outer segment structures h
Autor:
Patrick Ovando-Roche
The piggyBac transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells lines, including stem cells, without leaving behind any accessory DNA sequences like for example it happens in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f7a495275fa7e8ea70dacc1191f39109
https://doi.org/10.17504/protocols.io.bu56ny9e
https://doi.org/10.17504/protocols.io.bu56ny9e
Autor:
Robin R. Ali, Patrick Ovando-Roche, Alexander J. Smith, Rachael A. Pearson, Anastasios Georgiadis
Publikováno v:
Current Stem Cell Reports
Purpose of Review A major cause of visual disorders is dysfunction and/or loss of the light-sensitive cells of the retina, the photoreceptors. To develop better treatments for patients, we need to understand how inherited retinal disease mutations re
Autor:
Ricardo, Sanz, Patrick, Ovando-Roche, Namrata D, Udeshi, Steven A, Carr, Chee Yeun, Chung, Vikram, Khurana
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1948
In this chapter, we describe a novel ascorbate peroxidase (APEX)-based labeling method that in combination with mass spectrometry identifies proteins in the immediate vicinity of αSyn in living rat cortical neurons. To isolate these interactions, we
Autor:
Ricardo Sanz, Patrick Ovando-Roche, Vikram Khurana, Namrata D. Udeshi, Steven A. Carr, Chee Yeun Chung
Publikováno v:
Methods in Molecular Biology ISBN: 9781493991235
In this chapter, we describe a novel ascorbate peroxidase (APEX)-based labeling method that in combination with mass spectrometry identifies proteins in the immediate vicinity of αSyn in living rat cortical neurons. To isolate these interactions, we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f94fce4bb01d430bdfc923384c87a5e6
https://doi.org/10.1007/978-1-4939-9124-2_18
https://doi.org/10.1007/978-1-4939-9124-2_18
Autor:
Alexander J. Smith, Robin R. Ali, Matsuki Takaaki, Robert D. Sampson, Rachael A. Pearson, Anai Gonzalez-Cordero, Milan Fernando, Manjit S Mehat, Arifa Naeem, Patrick Ovando-Roche, Samuel J.I. Blackford, Kamil Kruczek, Shyamanga Borooah, Sophia-Martha Kleine Holthaus, Leticia Agundez, Debbie Goh, Magdalena Kloc, Emma L. West
Publikováno v:
Human gene therapy. 29(10)
Adeno-associated viral vectors are showing great promise as gene therapy vectors for a wide range of retinal disorders. To date, evaluation of therapeutic approaches has depended almost exclusively on the use of animal models. With recent advances in
Autor:
Patrick Ovando-Roche, Wei Cui
Ubiquitination is the first step of the ubiquitin-proteasome pathway that regulates cells for their homeostatic functions and is an enzymatic, protein post-translational modification process in which ubiquitin is transferred to a target protein subst
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::732496df9abfcf7105427f8e3acba55d
https://europepmc.org/articles/PMC5524179/
https://europepmc.org/articles/PMC5524179/
Publikováno v:
Europe PubMed Central
Telomere repeat binding factor 2 (TRF2) is a component of the shelterin complex that is known to bind and protect telomeric DNA, yet the detection of TRF2 in extra-telomeric regions of chromosomes suggests other roles for TRF2 besides telomere protec