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pro vyhledávání: '"Patrick Hublau"'
Interaction of mammalian sperm nuclear protamines and peptides derived thereof with immobilized zinc
Publikováno v:
International Journal of Peptide and Protein Research. 43:410-416
The interaction of mammalian and human protamines with zinc was studied by immobilized metal ion affinity chromatography (IMAC). The affinity of protamines containing blocked cysteine residues was found to correlate in part with the presence and numb
Autor:
Patrick Hublau, Bernard Sablonnière, Nathalie Dallery, Pierre Formstecher, Pierre-Marie Danze, Michel Dautrevaux
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1220:85-93
The hydrodynamic parameters of the retinoic-acid receptor from human myeloblastic leukemia HL-60 cells were accurately investigated. The ligand-bound retinoic-acid receptor (RAR) has a Stokes radius of 3.5 nm when analyzed by size-exclusion chromatog
Autor:
Roselyne Rousseaux-Prevost, Martha de Almeida, Pierre Jouannet, Jean Rousseaux, Pierre Sautiere, Patrick Hublau
Publikováno v:
Molecular Immunology. 29:895-902
The sera of vasectomized men and of patients with immune infertility were used to study the antigens and epitopes of sperm nuclear proteins that bind antibodies in these sera. No reaction with sperm histones was observed except for one serum. P1, P2
Publikováno v:
Molecular immunology. 28(9)
Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or
Publikováno v:
American Journal of Reproductive Immunology. 20:17-20
The auto-antibody response in infertile men was investigated by means of immunoenzymatic methods, dot-immunobinding assay (DIBA), and ELISA, using, as antigens, human sperm basic nuclear proteins. Comparison was made, for the same patients, with anti
Publikováno v:
Journal of gerontology. 44(6)
Polyclonal antibodies were raised against a synthetic peptide corresponding to a subsequence for the first 10 residues of the beta amyloid protein A4 (1-10 beta PA4). In an immunoperoxidase study of Alzheimer brain tissue, these antibodies immunostai