Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Patrick C. Cirino"'
Autor:
Rohan Kulkarni, Vijay Maranholkar, Nam Nguyen, Patrick C. Cirino, Richard C. Willson, Navin Varadarajan
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
Agonists of the stimulator of interferon genes (STING) pathway are being explored as potential immunotherapeutics for the treatment of cancer and as vaccine adjuvants for infectious diseases. Although chemical synthesis of 2′3’ - cyclic Guanosine
Externí odkaz:
https://doaj.org/article/3ffd210b80c846e1be8aa7892fce9a4c
Publikováno v:
Biosensors, Vol 14, Iss 7, p 325 (2024)
Microbial alkane degradation pathways provide biological routes for converting these hydrocarbons into higher-value products. We recently reported the functional expression of a methyl-alkylsuccinate synthase (Mas) system in Escherichia coli, allowin
Externí odkaz:
https://doaj.org/article/c2434577e60042d88d502ba1a62782e3
Publikováno v:
Microbial Cell Factories, Vol 18, Iss 1, Pp 1-9 (2019)
Abstract Background Salicylate can be biosynthesized from the common metabolic intermediate shikimate and has found applications in pharmaceuticals and in the bioplastics industry. While much metabolic engineering work focused on the shikimate pathwa
Externí odkaz:
https://doaj.org/article/ef512042d3784c4686ba7f0917e3699e
Publikováno v:
PLoS ONE, Vol 15, Iss 9 (2020)
The type I polyketide SF2487/A80577 (herein referred to as tetromadurin) is a polyether tetronate ionophore antibiotic produced by the terrestrial Gram-positive bacterium Actinomadura verrucosospora. Tetromadurin is closely related to the polyether t
Externí odkaz:
https://doaj.org/article/39e4d3e083864f168d6b77709c12503a
Publikováno v:
J Biotechnol
With continued progress in cell and gene therapies, there is an immediate need for exogenously tunable gene expression systems with safe and predictable behavior in specific human cell types. Here, we demonstrate the ability of the salicylic acid (SA
Publikováno v:
BioTechniques, Vol 59, Iss 3, Pp 157-161 (2015)
Iterative screening of expressed protein libraries using fluorescence-activated cell sorting (FACS) typically involves culturing the pooled clones after each sort. In these experiments, if cell viability is compromised by the sort conditions and/or e
Externí odkaz:
https://doaj.org/article/cbb0d637176f41c6b0c5464c8651fc4f
Publikováno v:
Crit Rev Biotechnol
Tools for tuning transcription in mammalian cells have broad applications, from basic biological discovery to human gene therapy. While precise control over target gene transcription via dosing with small molecules (drugs) is highly sought, the desig
Autor:
Ramon Gonzalez, Yixi Wang, Nam Tuan Nguyen, Seung Hwan Lee, Patrick C. Cirino, Qinxuan Wang, Jeremy A. May
Publikováno v:
Biotechnology and bioengineeringREFERENCES. 119(1)
In anoxic environments, microbial activation of alkanes for subsequent metabolism occurs most commonly through the addition of fumarate to a subterminal carbon, producing an alkylsuccinate. Alkylsuccinate synthases are complex, multi-subunit enzymes
Autor:
Matthew J Grisewood, Nathanael P Gifford, Robert J Pantazes, Ye Li, Patrick C Cirino, Michael J Janik, Costas D Maranas
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e75358 (2013)
OptZyme is a new computational procedure for designing improved enzymatic activity (i.e., kcat or kcat/KM) with a novel substrate. The key concept is to use transition state analogue compounds, which are known for many reactions, as proxies for the t
Externí odkaz:
https://doaj.org/article/9db33d589d5040eb9f21a47060d740a9
We previously described the design of triacetic acid lactone (TAL) biosensor ‘AraC-TAL1’, based on the AraC regulatory protein. Although useful as a tool to screen for enhanced TAL biosynthesis, this variant shows elevated background (leaky) expr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd022df22d374a38db578c61f4ccc7ad
https://doi.org/10.1101/2020.09.15.299107
https://doi.org/10.1101/2020.09.15.299107