Microscale Quantitative Analysis of Polyhydroxybutyrate in Prokaryotes Using IDMS

Autor: Mariana Itzel Velasco Alvarez, Angela ten Pierick, Patricia T. N. van Dam, Reza Maleki Seifar, Mark C. M. van Loosdrecht, S. Aljoscha Wahl

Publikováno v: Metabolites, Vol 7, Iss 2, p 19 (2017)

Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strai

Externí odkaz: https://doaj.org/article/91dcfccfdb324bc5ab847302178b0d45

Analysis of metabolites from the tricarboxylic acid cycle for yeast and bacteria samples using gas chromatography mass spectrometry

Autor: Reza Maleki Seifar, Angela ten Pierick, Patricia T. N. van Dam

Publikováno v: Seifar, R M, ten Pierick, A & van Dam, P T N 2018, Analysis of metabolites from the tricarboxylic acid cycle for yeast and bacteria samples using gas chromatography mass spectrometry . in M Giera (ed.), Clinical Metabolomics : methods and protocols . Methods in Molecular Biology, vol. 1730, Humana Press Inc, pp. 277-282 . https://doi.org/10.1007/978-1-4939-7592-1_20
Clinical Metabolomics: methods and protocols, 277-282
STARTPAGE=277;ENDPAGE=282;TITLE=Clinical Metabolomics
Methods in Molecular Biology ISBN: 9781493975914

We here explain step by step the implementation of gas chromatography coupled with tandem mass spectrometry for the quantitative analysis of intracellular metabolites from the tricarboxylic acid (TCA) cycle such as citrate, isocitrate, alpha-ketoglut

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6d3f3fac7396df2d17f0976828599a7
https://research.vu.nl/en/publications/da28e1b7-69ac-4eee-90a2-3ddd18d6d32b

Microscale quantitative analysis of polyhydroxybutyrate in prokaryotes using IDMS

Autor: Mark C.M. van Loosdrecht, S. Aljoscha Wahl, Reza M. Seifar, Mariana Itzel Velasco Alvarez, Patricia T. N. van Dam, Angela ten Pierick

Publikováno v: Metabolites, 7(2)
Metabolites, Vol 7, Iss 2, p 19 (2017)
Metabolites
Metabolites; Volume 7; Issue 2; Pages: 19

Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strai

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0605b08b5142eca0e78b4f67af63bd61
http://resolver.tudelft.nl/uuid:1430de82-5f33-42f3-ad10-066f2f2b2086