Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Patricia M, Ferrier"'
Autor:
Paul A. De Sousa, Arlene Ross, Wei Cui, Samena Aslam, Diana Wylie, Sarah Burl, Patricia M. Ferrier, Chris Denning, A. John Clark, Ian Wilmut
Publikováno v:
Nature Cell Biology. 5:535-538
Cultured primary cells exhibit a finite proliferative lifespan, termed the Hayflick limit. Cloning by nuclear transfer can reverse this cellular ageing process and can be accomplished with cultured cells nearing senescence. Here we describe nuclear t
Autor:
Andras Dinnyes, Ailsa Travers, Ian Wilmut, W. A. Ritchie, Judy Fletcher, J.R. Dobrinsky, Wim Bosma, Alison Ainslie, Alan Archibald, Paul A. De Sousa, David F. Albertini, John Bracken, Linda Harkness, June Bowering, Bianca Gasparrini, Paul Johnston, Tim King, Marjorie Ritchie, Jie Zhu, Patricia M. Ferrier
Publikováno v:
Biology of Reproduction. 66:642-650
To clone a pig from somatic cells, we first validated an electrical activation method for use on ovulated oocytes. We then evaluated delayed versus simultaneous activation (DA vs. SA) strategies, the use of 2 nuclear donor cells, and the use of cytos
Autor:
S Dhanjal, Ian Wilmut, Ram Mandalam, Jane Lebkowski, Linda Harkness, Judy Fletcher, Yogesh R Prasad, David G Brownstein, Joy D. A. Delhanty, John O. Gardner, Patricia M. Ferrier, Paul A. De Sousa, Paul Serhal, Joyce C. Harper
Publikováno v:
Fletcher, J M, Ferrier, P M, Gardner, J O, Harkness, L, Dhanjal, S, Serhal, P, Harper, J, Delhanty, J, Brownstein, D G, Prasad, Y R, Lebkowski, J, Mandalam, R, Wilmut, I & De Sousa, P A 2006, ' Variations in Humanized and Defined Culture Conditions Supporting Derivation of New Human Embryonic Stem Cell Lines ', Cloning and Stem Cells, vol. 8, no. 4, pp. 319-34 . https://doi.org/10.1089/clo.2006.8.319
The evolution of "humanized" (i.e., free of animal sourced reagents) and ultimately chemically defined culture systems for human embryo stem cell (hESC) isolation and culture is of importance to improving their efficacy and safety in research and the
Autor:
W. A. Ritchie, CB Clarke, Björn Oback, Ana Colette Maurício, Mário Sousa, Patricia M. Ferrier, Ricardo Ribas, Ian Wilmut, T. Chebotareva, E J Gallagher, Jane Taylor
Publikováno v:
Cloning and stem cells. 7(2)
In the present study, a zona-free nuclear transfer (NT) technique, which had been originally developed in cattle, was modified for the mouse. Steps involved in this approach include removing the zona pellucida and enucleating without a holding pipett
Autor:
Paul A, De Sousa, John R, Dobrinsky, Jie, Zhu, Alan L, Archibald, Alison, Ainslie, Wim, Bosma, June, Bowering, John, Bracken, Patricia M, Ferrier, Judy, Fletcher, Bianca, Gasparrini, Linda, Harkness, Paul, Johnston, Marjorie, Ritchie, William A, Ritchie, Ailsa, Travers, David, Albertini, Andras, Dinnyes, Timothy J, King, Ian, Wilmut
Publikováno v:
Biology of reproduction. 66(3)
To clone a pig from somatic cells, we first validated an electrical activation method for use on ovulated oocytes. We then evaluated delayed versus simultaneous activation (DA vs. SA) strategies, the use of 2 nuclear donor cells, and the use of cytos