Determining the structure of the bacterial voltage-gated sodium channel NaChBac embedded in liposomes by cryo electron tomography and subtomogram averaging

Autor: Shih-Ying Scott Chang, Patricia M. Dijkman, Simon A. Wiessing, Misha Kudryashev

Publikováno v: Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)

Abstract Voltage-gated sodium channels shape action potentials that propagate signals along cells. When the membrane potential reaches a certain threshold, the channels open and allow sodium ions to flow through the membrane depolarizing it, followed

Externí odkaz: https://doaj.org/article/feadb23b05ec4f4fbbf1f4588303e3c6

Dynamic tuneable G protein-coupled receptor monomer-dimer populations

Autor: Patricia M. Dijkman, Oliver K. Castell, Alan D. Goddard, Juan C. Munoz-Garcia, Chris de Graaf, Mark I. Wallace, Anthony Watts

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018)

Evidence suggests oligomerisation of G protein-coupled receptors in membranes, but this is controversial. Here, authors use single-molecule and ensemble FRET, and spectroscopy to show that the neurotensin receptor 1 forms multiple dimer conformations

Externí odkaz: https://doaj.org/article/28f2d38675864c7fa3f3f5c8d36e8dc1

Conformational dynamics of a G protein-coupled receptor helix 8 in lipid membranes

Autor: Antonio J. Costa-Filho, Patricia M. Dijkman, Juan C. Muñoz-García, Anthony Watts, Rosana I. Reis, Steven Lavington, Daniel Yin, Phillip J. Stansfeld, Patricia S. Kumagai

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Science Advances

Helix 8 of the GPCR neurotensin receptor 1 is stabilised by specific lipid-protein interactions.
G protein–coupled receptors (GPCRs) are the largest and pharmaceutically most important class of membrane proteins encoded in the human genome, ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::483a0810dd023e844410834f47a9deb0

Single molecule fluorescence for membrane proteins

Autor: Oliver Kieran Castell, Alan D. Goddard, Daniel N. Wiseman, Patricia M. Dijkman

Publikováno v: Methods (San Diego, Calif.). 147

The cell membrane is a complex milieu of lipids and proteins. In order to understand the behaviour of individual molecules is it often desirable to examine them as purified components in in vitro systems. Here, we detail the creation and use of dropl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::961b48f6b3b94a8eb97246cd9ed2386c
https://pubmed.ncbi.nlm.nih.gov/29857189

Dynamic tuneable G protein-coupled receptor monomer-dimer populations

Autor: Patricia M. Dijkman, Chris de Graaf, Juan C. Muñoz-García, Anthony Watts, Alan D. Goddard, Mark I. Wallace, Oliver Kieran Castell

Publikováno v: Nature Communications
Nature Communications, 9(1):1710. Nature Publishing Group
Dijkman, P M, Castell, O K, Goddard, A D, Munoz-Garcia, J C, de Graaf, C, Wallace, M I & Watts, A 2018, ' Dynamic tuneable G protein-coupled receptor monomer-dimer populations ', Nature Communications, vol. 9, 1710 . https://doi.org/10.1038/s41467-018-03727-6
Dijkman, P M, Castell, O K, Goddard, A D, Munoz-Garcia, J C, De Graaf, C, Wallace, M I & Watts, A 2018, ' Dynamic tuneable G protein-coupled receptor monomer-dimer populations ', Nature Communications, vol. 9, no. 1, 1710 . https://doi.org/10.1038/s41467-018-03727-6
Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018)

G protein-coupled receptors (GPCRs) are the largest class of membrane receptors, playing a key role in the regulation of processes as varied as neurotransmission and immune response. Evidence for GPCR oligomerisation has been accumulating that challe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d4b1486665ab237fa2540c902fc1b80
https://pubmed.ncbi.nlm.nih.gov/29703992