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pro vyhledávání: '"Patricia Buckley Ahrens"'
Publikováno v:
Developmental Biology. 60:69-82
Cells from wing buds of varying-stage chick embryos were dissociated and grown in culture to test their capacity for cartilage differentiation. Micro-mass cultures were initiated with a cell layer greater than confluency, which occupied a restricted
Publikováno v:
Journal of Experimental Zoology. 202:375-388
Day 3 mass muscle cultures incorporated 35SO4= and 3H· glucosamine into specific glycosaminoglycans that were identified by a variety of methods (enzyme susceptibility, nitrous acid degradation, electrophoresis, thin layer chromatography). More than
Publikováno v:
In vitro. 14(1)
Tissue-culture methods can be used to test the developmental capacity of embryonic cells. In micro-mass cultures, derived from wing cells of stages 21 through 24 chick embryos, aggregates of cells form and then differentiate into cartilage nodules, a
Publikováno v:
Differentiation; research in biological diversity. 15(3)
In the present study the level of cAMP was measured during in vitro chondrogenesis of wing mesenchyme of stage 24 chick embryos and was found to increase significantly from 6.3 pmol/mg protein at the end of the first day of culture to 9.7 pmol/mg pro
Publikováno v:
Developmental biology. 69(2)
A quantitative comparison (i.e., number of cartilage nodules) of cartilage differentiation was made between micromass cell cultures prepared with cells from different locations (core vs periphery) within prechondrogenic chick wing buds. Wing bud core
Publikováno v:
Developmental biology. 83(1)
The responsiveness of high-density cell cultures (micromass) prepared from different stages or different regions of chick embryonic wings to treatment with dibutyryl cyclic AMP (dbcAMP) and related compounds is used to define cellular heterogeneity a