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The application of flavin-dependent halogenases is hampered by their lack of stability under reaction conditions. However, first attempts to improve halogenase stability by error-prone PCR have resulted in mutants with higher temperature stability. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ff030f433e68713d897cba76c3a5b63e
https://doi.org/10.1016/bs.mie.2016.03.022
https://doi.org/10.1016/bs.mie.2016.03.022
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Autor:
Glueck D; Molecular Biophysics, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.; Biophysics, Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, Humboldtstr. 50/III, Graz, 8010, Austria.; Field of Excellence BioHealth, University of Graz, Graz, Austria.; BioTechMed-Graz, Graz, Austria., Grethen A; Molecular Biophysics, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany., Das M; Molecular Biophysics, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany., Mmeka OP; Molecular Biophysics, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.; Department of Chemistry, University of Ibadan, Ibadan, 200284, Nigeria., Patallo EP; Molecular Biophysics, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany., Meister A; HALOmem and Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120, Halle (Saale), Germany., Rajender R; Human Biology, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany., Kins S; Human Biology, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany., Räschle M; Molecular Genetics, Technische Universität Kaiserslautern (TUK), Paul-Ehrlich-Str. 24, 67663, Kaiserslautern, Germany., Victor J; Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany., Chu C; Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany., Etzkorn M; Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany., Köck Z; Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt/Main, Max-von-Laue-Str. 9, 60438, Frankfurt/Main, Germany., Bernhard F; Centre for Biomolecular Magnetic Resonance, Institute for Biophysical Chemistry, Goethe University of Frankfurt/Main, Max-von-Laue-Str. 9, 60438, Frankfurt/Main, Germany., Babalola JO; Department of Chemistry, University of Ibadan, Ibadan, 200284, Nigeria., Vargas C; Molecular Biophysics, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.; Biophysics, Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, Humboldtstr. 50/III, Graz, 8010, Austria.; Field of Excellence BioHealth, University of Graz, Graz, Austria.; BioTechMed-Graz, Graz, Austria., Keller S; Molecular Biophysics, Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.; Biophysics, Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, Humboldtstr. 50/III, Graz, 8010, Austria.; Field of Excellence BioHealth, University of Graz, Graz, Austria.; BioTechMed-Graz, Graz, Austria.
Publikováno v:
Small (Weinheim an der Bergstrasse, Germany) [Small] 2022 Nov; Vol. 18 (47), pp. e2202492. Date of Electronic Publication: 2022 Oct 13.
Autor:
Das M; Department of Bionanotechnology, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 155-88, Korea.; Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663 Kaiserslautern, Germany., Mahler F; Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663 Kaiserslautern, Germany., Hariharan P; Department of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, United States., Wang H; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305, United States., Du Y; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305, United States., Mortensen JS; Department of Neuroscience, University of Copenhagen, Copenhagen DK-2200, Denmark., Patallo EP; Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663 Kaiserslautern, Germany., Ghani L; Department of Bionanotechnology, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 155-88, Korea., Glück D; Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663 Kaiserslautern, Germany., Lee HJ; Department of Bionanotechnology, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 155-88, Korea., Byrne B; Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom., Loland CJ; Department of Neuroscience, University of Copenhagen, Copenhagen DK-2200, Denmark., Guan L; Department of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, United States., Kobilka BK; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305, United States., Keller S; Technische Universität Kaiserslautern (TUK), Erwin-Schrödinger-Str. 13, 67663 Kaiserslautern, Germany.; Institute of Molecular Biosciences (IMB), NAWI Graz, University of Graz, Humboldtstr. 50/III, 8010 Graz, Austria.; Field of Excellence BioHealth, University of Graz, 8010 Graz, Austria.; BioTechMed-Graz, Graz, Austria., Chae PS; Department of Bionanotechnology, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 155-88, Korea.
Publikováno v:
Journal of the American Chemical Society [J Am Chem Soc] 2020 Dec 23; Vol. 142 (51), pp. 21382-21392. Date of Electronic Publication: 2020 Dec 14.
Autor:
Neumann M; Faculty of Biology, Institute of Botany, Technische Universität Dresden, 01062, Dresden, Germany., Prahl S; Vita34 AG, 04103, Leipzig, Germany., Caputi L; Department of Natural Product Synthesis, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany., Hill L; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom., Kular B; John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom., Walter A; Faculty of Biology, Institute of Botany, Technische Universität Dresden, 01062, Dresden, Germany., Patallo EP; Biochemistry, Technische Universität Dresden, 01062, Dresden, Germany., Milbredt D; Biochemistry, Technische Universität Dresden, 01062, Dresden, Germany., Aires A; Centre for the Research and Technology for Agro-Environment and Biological Sciences, CITAB, University of Trás-os-Montes e Alto Douro, 5001-801, Vila Real, Portugal., Schöpe M; Vita34 AG, 04103, Leipzig, Germany., O'Connor S; Department of Natural Product Synthesis, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany., van Pée KH; Biochemistry, Technische Universität Dresden, 01062, Dresden, Germany., Ludwig-Müller J; Faculty of Biology, Institute of Botany, Technische Universität Dresden, 01062, Dresden, Germany. Electronic address: Jutta.Ludwig-Mueller@tu-dresden.de.
Publikováno v:
Phytochemistry [Phytochemistry] 2020 Jul; Vol. 175, pp. 112371. Date of Electronic Publication: 2020 Apr 10.
Autor:
Milbredt D; Professur für Allgemeine Biochemie, Fachrichtung Chemie und Lebensmittelchemie, Technische Universität Dresden, 01062, Dresden, Germany., Patallo EP; Professur für Allgemeine Biochemie, Fachrichtung Chemie und Lebensmittelchemie, Technische Universität Dresden, 01062, Dresden, Germany., van Pée KH; Professur für Allgemeine Biochemie, Fachrichtung Chemie und Lebensmittelchemie, Technische Universität Dresden, 01062, Dresden, Germany. karl-heinz.vanpee@chemie.tu-dresden.de.
Publikováno v:
Chembiochem : a European journal of chemical biology [Chembiochem] 2016 Oct 04; Vol. 17 (19), pp. 1859-1864. Date of Electronic Publication: 2016 Aug 17.
Autor:
van Pée KH; Allgemeine Biochemie, TU Dresden, Dresden, Germany. Electronic address: karl-heinz.vanpee@chemie.tu-dresden.de., Milbredt D; Allgemeine Biochemie, TU Dresden, Dresden, Germany., Patallo EP; Allgemeine Biochemie, TU Dresden, Dresden, Germany., Weichold V; Allgemeine Biochemie, TU Dresden, Dresden, Germany., Gajewi M; Allgemeine Biochemie, TU Dresden, Dresden, Germany.
Publikováno v:
Methods in enzymology [Methods Enzymol] 2016; Vol. 575, pp. 65-92. Date of Electronic Publication: 2016 May 06.