Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Pascal Michalon"'
Publikováno v:
Journal of Membrane Science. 147:95-107
An artificial glucokinase/phosphatase porous membrane separating two unequal compartments of a diffusion cell was used to pump glucose from one compartment against the concentration gradient of the opposite one. Our results mainly demonstrate that, a
Publikováno v:
Journal of Membrane Science. 144:237-250
Publikováno v:
Journal of Membrane Science. 134:85-99
An approach to the mechanism which may govern translocation and active transport system is presented. Two artificial enzyme membranes with immobilized kinase and immobilized phosphatase, respectively, were used close together to separate two unequal
Publikováno v:
European Journal of Biochemistry. 216:387-394
The sensitive electric-birefringence method was used to reveal structural differences between the soluble chromatin of procyclic Trypanosoma brucei brucei and the chromatin of the higher eukaryotes. The orientation of the nucleosomal chains and the p
Publikováno v:
Analytica Chimica Acta. 263:85-91
An enzymatic biosensor for glycerol and glycerol-3-phosphate was designed, based on the co-immobilization of two enzymes, glycerol kinase and glycerol-3-phosphate oxidase, on a nylon membrane activated by a process recently reported for nylon balls.
Publikováno v:
Enzyme and microbial technology. 15(3)
Water-insoluble nucleases were prepared by immobilizing the endonuclease from S. aureus onto the surface of nylon-66 and polystyrene spheres. The activation phase of the synthetic supports was optimized to define optimal conditions of pH, temperature
Publikováno v:
Acta tropica. 52(1)
Soluble chromatin of Trypanosoma brucei brucei procyclic culture forms was submitted to digestion with free or immobilized trypsin. Digestion with trypsin in salt solutions of low and high ionic strengths generated characteristic sets of limit histon
Autor:
Marie-Françoise Hacques, Albert Ville, Roger Couturier, Guy Favre-Bonvin, Pascal Michalon, Christian Marion
Publikováno v:
Biochemical and biophysical research communications. 167(1)
A new method consisting of a two-step activation was developed in order to covalently immobilize enzymes on calibrate nylon 66 spheres. This efficient method associates for the first time peptide bond cleavage and O-alkylation of the support. Optimal