Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Pascal Egloff"'
Autor:
Mathieu Botte, Dongchun Ni, Stephan Schenck, Iwan Zimmermann, Mohamed Chami, Nicolas Bocquet, Pascal Egloff, Denis Bucher, Matilde Trabuco, Robert K. Y. Cheng, Janine D. Brunner, Markus A. Seeger, Henning Stahlberg, Michael Hennig
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Lateral opening of the LptDE transporter in the outer membrane of Gram-negative bacteria is necessary for insertion of lipopolysaccharides. Here, Botte et al. report a cryo-EM structure of a partially opened LptDE transporter, in complex with rigid c
Externí odkaz:
https://doaj.org/article/25e3d626c5e9434d8732b67049815a5b
Autor:
Mattia Deluigi, Lena Morstein, Matthias Schuster, Christoph Klenk, Lisa Merklinger, Riley R. Cridge, Lazarus A. de Zhang, Alexander Klipp, Santiago Vacca, Tasneem M. Vaid, Peer R. E. Mittl, Pascal Egloff, Stefanie A. Eberle, Oliver Zerbe, David K. Chalmers, Daniel J. Scott, Andreas Plückthun
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
This study reports the X-ray structure of the α1B-adrenergic G protein-coupled receptor bound to an inverse agonist, and unveils key determinants of subtype-selective ligand binding that may help the design of aminergic drugs with fewer side-effects
Externí odkaz:
https://doaj.org/article/9eae3c81d8e34aff9e67ac17ad3df5fe
Autor:
Cedric A. J. Hutter, M. Hadi Timachi, Lea M. Hürlimann, Iwan Zimmermann, Pascal Egloff, Hendrik Göddeke, Svetlana Kucher, Saša Štefanić, Mikko Karttunen, Lars V. Schäfer, Enrica Bordignon, Markus A. Seeger
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
ABC exporters hydrolyze ATP to pump substrates across membranes, but critical steps of the transport mechanism remain poorly understood. Here, the authors solve the crystal structure of outward-facing TM287/288 with the help of a state-specific sybod
Externí odkaz:
https://doaj.org/article/10e4f9ba103940ba80927107a3cf8a89
Autor:
Iwan Zimmermann, Pascal Egloff, Cedric AJ Hutter, Fabian M Arnold, Peter Stohler, Nicolas Bocquet, Melanie N Hug, Sylwia Huber, Martin Siegrist, Lisa Hetemann, Jennifer Gera, Samira Gmür, Peter Spies, Daniel Gygax, Eric R Geertsma, Roger JP Dawson, Markus A Seeger
Publikováno v:
eLife, Vol 7 (2018)
Mechanistic and structural studies of membrane proteins require their stabilization in specific conformations. Single domain antibodies are potent reagents for this purpose, but their generation relies on immunizations, which impedes selections in th
Externí odkaz:
https://doaj.org/article/0128ad13246b41d488888b761db2ce68
Autor:
Mario Schubert, Silvia Bleuler-Martinez, Alex Butschi, Martin A. Wälti, Pascal Egloff, Katrin Stutz, Shi Yan, Mayeul Collot, Jean-Maurice Mallet, Iain B. H. Wilson, Michael O. Hengartner, Markus Aebi, Frédéric H.-T. Allain, Markus Künzler
Publikováno v:
PLoS Pathogens, Vol 8, Iss 8 (2012)
Externí odkaz:
https://doaj.org/article/c0c170b59efc4fe98329ef68d86e3dd4
Autor:
Mario Schubert, Silvia Bleuler-Martinez, Alex Butschi, Martin A Wälti, Pascal Egloff, Katrin Stutz, Shi Yan, Mayeul Collot, Jean-Maurice Mallet, Iain B H Wilson, Michael O Hengartner, Markus Aebi, Frédéric H-T Allain, Markus Künzler
Publikováno v:
PLoS Pathogens, Vol 8, Iss 5, p e1002706 (2012)
Discrimination between self and non-self is a prerequisite for any defence mechanism; in innate defence, this discrimination is often mediated by lectins recognizing non-self carbohydrate structures and so relies on an arsenal of host lectins with di
Externí odkaz:
https://doaj.org/article/45c7d5b3702e41178f59d545fd19aa0b
Autor:
Pascal Egloff, Markus A. Seeger, Saša Štefanić, Lea M. Hürlimann, Cedric A. J. Hutter, Enrica Bordignon, Hendrik Göddeke, Mikko Karttunen, Lars V. Schäfer, Iwan Zimmermann, M. Hadi Timachi, Svetlana Kucher
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications
Nature Communications
ABC exporters harness the energy of ATP to pump substrates across membranes. Extracellular gate opening and closure are key steps of the transport cycle, but the underlying mechanism is poorly understood. Here, we generated a synthetic single domain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::653508ffd7b661b4320addf009de7472
http://link.springer.com/article/10.1038/s41467-019-09892-6
http://link.springer.com/article/10.1038/s41467-019-09892-6
Autor:
Robert K. Y. Cheng, Mohamed Chami, Matilde Cardoso Trabuco, Stephan Schenck, Janine D. Brunner, Henning Stahlberg, Michael Hennig, Denis Bucher, Markus A. Seeger, Pascal Egloff, Nicolas Bocquet, Iwan Zimmermann, Mathieu Botte, Dongchun Ni
Lipopolysaccharides (LPS) are major constituents of the extracellular leaflet in the bacterial outer membrane and form an effective physical barrier for environmental threats and for antibiotics in Gram-negative bacteria 1. The last step of LPS inser
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4301c1aaeea1a661029ab33ce3c58b2
https://doi.org/10.1101/2021.03.23.436624
https://doi.org/10.1101/2021.03.23.436624
Autor:
Benedikt T. Kuhn, Iwan Zimmermann, Pascal Egloff, Lea M. Hürlimann, Cedric A.J. Hutter, Christian Miscenic, Roger J.P. Dawson, Markus A. Seeger, Eric R. Geertsma
The selective immobilization of proteins represents an essential step in the selection of binding proteins such as antibodies. The immobilization strategy determines how the target protein is presented to the binders and thereby directly affects the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8e91f44c161e81d63919ee447e88d400
https://doi.org/10.17504/protocols.io.bpinmkde
https://doi.org/10.17504/protocols.io.bpinmkde
Autor:
Philipp Bräuer, Pascal Egloff, Simon Newstead, Markus A. Seeger, Eric R. Geertsma, Benedikt T Kuhn, Roger J. P. Dawson, Iwan Zimmermann, Cedric A. J. Hutter
Here, we provide a protocol to generate synthetic nanobodies, known as sybodies, against any purified protein or protein complex within a 3-week period. Unlike methods that require animals for antibody generation, sybody selections are carried out en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60fb504f98721c103baafee2d8c5815
https://doi.org/10.5167/uzh-190529
https://doi.org/10.5167/uzh-190529