Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Parker E. Deal"'
Autor:
Molly J. Kirk, Brittany R. Benlian, Yifu Han, Arya Gold, Ashvin Ravi, Parker E. Deal, Rosana S. Molina, Mikhail Drobizhev, Dion Dickman, Kristin Scott, Evan W. Miller
Publikováno v:
Frontiers in Neuroscience, Vol 15 (2021)
We combine a chemically-synthesized, voltage-sensitive fluorophore with a genetically encoded, self-labeling enzyme to enable voltage imaging in Drosophila melanogaster. Previously, we showed that a rhodamine voltage reporter (RhoVR) combined with th
Externí odkaz:
https://doaj.org/article/8bb0c427fcf548cfaf73e51d8ca6a5f4
Autor:
Michelina Kierzek, Parker E Deal, Evan W Miller, Shatanik Mukherjee, Dagmar Wachten, Arnd Baumann, U Benjamin Kaupp, Timo Strünker, Christoph Brenker
Publikováno v:
eLife, Vol 10 (2021)
Fluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (Vm) are invaluable tools to study cellular signaling pathways. Here, we introduce a novel technique for simultane
Externí odkaz:
https://doaj.org/article/ff6c6b36dc74490c9dc0a6d1e5cee781
Autor:
Pavel E. Z. Klier, Anneliese M. M. Gest, Julia G. Martin, Ryan Roo, Marisol X. Navarro, Lauren Lesiak, Parker E. Deal, Neville Dadina, Jonathan Tyson, Alanna Schepartz, Evan W. Miller
Publikováno v:
Journal of the American Chemical Society. 144:12138-12146
Electrical potential differences across lipid bilayers play foundational roles in cellular physiology. Plasma membrane voltage is the most widely studied; however, the bilayers of organelles like mitochondria, lysosomes, nuclei, and the endoplasmic r
Autor:
Kiarash Shamardani, Hillel Adesnik, Ashley K. Nensel, Kayli N. Martinez, Gloria Ortiz, Evan W. Miller, Pei Liu, Parker E. Deal
Publikováno v:
RSC Chemical Biology
We describe the design, synthesis, and application of voltage-sensitive silicon rhodamines. Based on the Berkeley Red Sensor of Transmembrane potential, or BeRST, scaffold, the new dyes possess an isomeric molecular wire for improved alignment in the
Autor:
Vikram R Muller, Pei Liu, Kiarash Shamardani, Hillel Adesnik, Parker E. Deal, Evan W. Miller, Sarah H. Al-Abdullatif
Publikováno v:
J Am Chem Soc
Voltage-sensitive fluorophores enable the direct visualization of membrane potential changes in living systems. To pair the speed and sensitivity of chemical synthesized fluorescent indicators with cell-type specific genetic methods, we here develop
Autor:
Michelina Kierzek, Parker E Deal, Evan W Miller, Shatanik Mukherjee, Dagmar Wachten, Arnd Baumann, U Benjamin Kaupp, Timo Strünker, Christoph Brenker
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::993db41f441434c47918cf9cb8b6b881
https://doi.org/10.7554/elife.63129.sa2
https://doi.org/10.7554/elife.63129.sa2
Autor:
Ashvin Ravi, Molly J. Kirk, Arya Gold, Yifu Han, Parker E. Deal, Evan W. Miller, Brittany R. Benlian, Mikhail Drobizhev, Kristin Scott, Rosana S. Molina, Dion Dickman
We combine a chemically-synthesized, voltage-sensitive fluorophore with a genetically encoded, self-labeling enzyme to enable voltage imaging in Drosophila melanogaster. Previously, we showed that a rhodamine voltage reporter (RhoVR) combined with th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac73e32fbfda7f9704646472796b3458
https://doi.org/10.1101/2021.08.05.455279
https://doi.org/10.1101/2021.08.05.455279
Publikováno v:
Journal of molecular biology, vol 433, iss 17
Leak currents, defined as voltage and time independent flows of ions across cell membranes, are central to cellular electrical excitability control. The K2P (KCNK) potassium channel class comprises an ion channel family that produces potassium leak c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::083cbf3119ec46f634eae81b29c4fcce
https://escholarship.org/uc/item/33762049
https://escholarship.org/uc/item/33762049
Autor:
Parker E. Deal, Jonathan S. Marvin, Loren L. Looger, Abbas Kazemipour, Jonathan P. King, Daniel Flickinger, Ondrej Novak, Jeong Jun Kim, Sarah H. Al-Abdullatif, Eric R. Schreiter, Philip M. Borden, Ahmed S. Abdelfattah, Shaul Druckmann, Kaspar Podgorski, Karel Svoboda, Evan W. Miller
Publikováno v:
Nature methods
Point-scanning two-photon microscopy enables high-resolution imaging within scattering specimens such as the mammalian brain, but sequential acquisition of voxels fundamentally limits its speed. We developed a two-photon imaging technique that scans
Publikováno v:
Journal of molecular biology
Leak currents, defined as voltage and time independent flows of ions across cell membranes, are central to cellular electrical excitability control. The K2P (KCNK) potassium channel class comprises an ion channel family that produces potassium leak c