Zobrazeno 1 - 10
of 236
pro vyhledávání: '"Paolo, Bartolini"'
Molecular Cloning and AlphaFold Modeling of Thyrotropin (ag-TSH) From the Amazonian Fish Pirarucu ()
Autor:
Renan Passos Freire, Jorge Enrique Hernandez-Gonzalez, Eliana Rosa Lima, Miriam Fussae Suzuki, João Ezequiel de Oliveira, Lucas Simon Torati, Paolo Bartolini, Carlos Roberto Jorge Soares
Publikováno v:
Bioinformatics and Biology Insights, Vol 17 (2023)
Arapaima gigas , known as Pirarucu in Brazil, is one of the largest freshwater fish in the world. Some individuals could reach 3 m in length and weight up to 200 kg. Due to extinction risks and its economic value, the species has been a focus for pre
Externí odkaz:
https://doaj.org/article/c0467bd221a845458086c8209acc16fa
Autor:
Sara Catalini, Viviane Lutz-Bueno, Mattia Usuelli, Michael Diener, Andrea Taschin, Paolo Bartolini, Paolo Foggi, Marco Paolantoni, Raffaele Mezzenga, Renato Torre
Publikováno v:
iScience, Vol 25, Iss 7, Pp 104586- (2022)
Summary: Reactive amyloid oligomers are responsible for cytotoxicity in amyloid pathologies and because of their unstable nature characterizing their behavior is a challenge. The physics governing the self-assembly of proteins in crowded conditions i
Externí odkaz:
https://doaj.org/article/a4e88d956d2d475f9bf85c253118fa34
Autor:
Miriam F. Suzuki, Larissa A. Almeida, Stephanie A. Pomin, Felipe D. Silva, Renan P. Freire, João E. Oliveira, Regina Affonso, Carlos R. J. Soares, Paolo Bartolini
Publikováno v:
AMB Express, Vol 11, Iss 1, Pp 1-12 (2021)
Abstract The human prolactin antagonist Δ1-11-G129R-hPRL is a 21.9 kDa recombinant protein with 188 amino acids that downregulates the proliferation of a variety of cells expressing prolactin receptors. Periplasmic expression of recombinant proteins
Externí odkaz:
https://doaj.org/article/1d531840760f44019d5cc75e57be97cd
Autor:
Miriam Fussae Suzuki, João Ezequiel Oliveira, Renata Damiani, Eliana Rosa Lima, Kleicy Cavalcante Amaral, Anderson Maikon de Souza Santos, Geraldo Santana Magalhães, Leonardo Perez Faverani, Luis Antonio Violin Dias Pereira, Fabiana Medeiros Silva, Paolo Bartolini
Publikováno v:
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
Abstract Commercially available preparations of methionyl-human BMP-2 and CHO-derived hBMP-2, which belongs to the transforming growth factor β (TGF-β) superfamily, were used for a complete characterization. This protein is an extremely efficient o
Externí odkaz:
https://doaj.org/article/ab91ab220a814158b60e20c44603ebe1
Autor:
Eliana Rosa Lima, Renan Passos Freire, Miriam Fussae Suzuki, João Ezequiel Oliveira, Vanessa Luna Yosidaki, Cibele Nunes Peroni, Thaís Sevilhano, Moisés Zorzeto, Lucas Simon Torati, Carlos Roberto Jorge Soares, Igor Daniel de Miranda Lima, Thales Kronenberger, Vinicius Gonçalves Maltarollo, Paolo Bartolini
Publikováno v:
Biomolecules, Vol 13, Iss 1, p 158 (2023)
In a previous work, the common gonadotrophic hormone α-subunit (ag-GTHα), the ag-FSH β- and ag-LH β-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also c
Externí odkaz:
https://doaj.org/article/c636eb1896ea4568b3faaabff5818cc1
Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Autor:
Felipe D. Silva, João E. Oliveira, Renan P. Freire, Miriam F. Suzuki, Carlos R. Soares, Paolo Bartolini
Publikováno v:
AMB Express, Vol 9, Iss 1, Pp 1-11 (2019)
Abstract Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation. The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cell
Externí odkaz:
https://doaj.org/article/497c47353537435b91bfc3a50307d0e9
Autor:
João E. Oliveira, Miriam F. Suzuki, Renata Damiani, Eliana R. Lima, Kleicy C. Amaral, Anderson M. S. Santos, Geraldo S. Magalhães, Leonardo P. Faverani, Luís A. V. D. Pereira, Paolo Bartolini
Publikováno v:
Cells, Vol 10, Iss 12, p 3525 (2021)
Human BMP-2, a homodimeric protein that belongs to the TGF- β family, is a recognized osteoinductor due to its capacity of inducing bone regeneration and ectopic bone formation. The administration of its recombinant form is an alternative to autolog
Externí odkaz:
https://doaj.org/article/6a703638529f47ac9dde968badaea893
Autor:
Rosa Maria Chura-Chambi, Cleide Mara Rosa da Silva, Lennon Ramos Pereira, Paolo Bartolini, Luis Carlos de Souza Ferreira, Ligia Morganti
Publikováno v:
PLoS ONE, Vol 14, Iss 1, p e0211162 (2019)
In this study we evaluated the association of high hydrostatic pressure (HHP) and alkaline pH as a minimally denaturing condition for the solubilization of inclusion bodies (IBs) generated by recombinant proteins expressed by Escherichia coli strains
Externí odkaz:
https://doaj.org/article/370b146afb764e54a517efba0c4b6052
Autor:
Eliana Rosa Lima, Claudia Regina Cecchi, Eliza Higuti, Gustavo Protasio Pacheco de Jesus, Alissandra Moura Gomes, Enio Aparecido Zacarias, Paolo Bartolini, Cibele Nunes Peroni
Publikováno v:
Molecules, Vol 25, Iss 21, p 5034 (2020)
Previous non-viral gene therapy was directed towards two animal models of dwarfism: Immunodeficient (lit/scid) and immunocompetent (lit/lit) dwarf mice. The former, based on hGH DNA administration into muscle, performed better, while the latter, a ho
Externí odkaz:
https://doaj.org/article/1c3d4442b89c46bf91ca700c8cb5db34
Autor:
Marie Plazanet, Jordanka Tasseva, Paolo Bartolini, Andrea Taschin, Renato Torre, Christèle Combes, Christian Rey, Alessandro Di Michele, Mariana Verezhak, Aurelien Gourrier
Publikováno v:
PLoS ONE, Vol 13, Iss 8, p e0201745 (2018)
Because of the importance of bone in the biomedical, forensic and archaeological contexts, new investigation techniques are constantly required to better characterize bone ultrastructure. In the present paper, we provide an extended investigation of
Externí odkaz:
https://doaj.org/article/7de201702be1441b9b3384dd7020c5e3