Two Sets of Piwi Proteins Are Involved in Distinct sRNA Pathways Leading to Elimination of Germline-Specific DNA

Autor: Dominique I. Furrer, Estienne C. Swart, Matthias F. Kraft, Pamela Y. Sandoval, Mariusz Nowacki

Publikováno v: Cell Reports, Vol 20, Iss 2, Pp 505-520 (2017)

Piwi proteins and piRNAs protect eukaryotic germlines against the spread of transposons. During development in the ciliate Paramecium, two Piwi-dependent sRNA classes are involved in the elimination of transposons and transposon-derived DNA: scan RNA

Externí odkaz: https://doaj.org/article/9bc960ffac0c4664aea00a7aef70105c

Fluid Brain Glycolysis: Limits, Speed, Location, Moonlighting, and the Fates of Glycogen and Lactate

Autor: Daniela Rauseo, Yasna Contreras-Baeza, Alejandro San Martín, Sharin Valdivia, Felipe Baeza-Lehnert, Alex Galaz, Pamela Y. Sandoval, Robinson Arce-Molina, L. Felipe Barros, Iván Ruminot

Publikováno v: Neurochemical Research. 45:1328-1334

Glycolysis is the core of intermediate metabolism, an ancient pathway discovered in the heydays of classic biochemistry. A hundred years later, it remains a matter of active research, clinical interest and is not devoid of controversy. This review ex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d1a534e5f80c31b9019357eab2ae707
https://doi.org/10.1007/s11064-020-03005-2

A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier MPC

Autor: Stefanie Schirmeier, Alex Galaz, L. F. Barros, Karin Alegría, Francisca Cortés-Molina, Pamela Y. Sandoval, Robinson Arce-Molina, Alejandro San Martín

Publikováno v: eLife
eLife, Vol 9 (2020)

Mitochondria generate ATP and building blocks for cell growth and regeneration, using pyruvate as the main substrate. Here we introduce PyronicSF, a user-friendly GFP-based sensor of improved dynamic range that enables real-time subcellular quantitat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e10b32c2654a5c667a5dcefe4ceaf9e9
https://doi.org/10.1101/611806

MitoToxy Assay: a novel cell-based method for the assessment of metabolic toxicity in a multiwell plate format using a lactate FRET nanosensor, Laconic

Autor: Pamela Y. Sandoval, Robinson Arce-Molina, Alejandro San Martín, Karin Alegría, L. F. Barros, Sebastián Ceballo, Yasna Contreras-Baeza

Publikováno v: PLoS ONE
PLoS ONE, Vol 14, Iss 10, p e0224527 (2019)

Mitochondrial toxicity is a primary source of pre-clinical drug attrition. Methods that detect mitochondrial toxicity as early as possible during the drug development process are required. Here we introduce a new method for detecting mitochondrial to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a87c0787315730b5f7d78347a2845725
https://doi.org/10.1101/583096

Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination

Autor: Estienne C. Swart, Miroslav Arambasic, Mariusz Nowacki, Michael Ignarski, Pamela Y. Sandoval, Aditi Singh

Publikováno v: Nucleic Acids Research
Ignarski, Michael; Singh, Aditi; Swart, Estienne Carl; Arambasic, Miroslav; Sandoval, Pamela Yohanna de Lourdes; Nowacki, Mariusz (2014). Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination. Nucleic acids research, 42(19), pp. 11952-11964. Information Retrieval Ltd. 10.1093/nar/gku874

Genome-wide DNA remodelling in the ciliate Paramecium is ensured by RNA-mediated trans-nuclear crosstalk between the germline and the somatic genomes during sexual development. The rearrangements include elimination of transposable elements, minisate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8fbe9770a5c5da68aac301a1d014aca
https://doi.org/10.1093/nar/gku874