Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Pamela Holzlöhner"'
Autor:
Natalia Maier, Monique Butze, Burkhard Micheel, Gabriele Heidicke, Erik Schliebs, Jonas Füner, Katja Hanack, Pamela Holzlöhner, Nicole Hebel
Camelids possess antibodies with a conventional four-chain structure consisting of two heavy and two light chains (of subclass IgG1) but further they also generate heavy-chain only antibodies (of subclass IgG2 and 3) which are fully functional in ant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::088364cd2960961e7743f84d9ebe2367
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/53444
https://publishup.uni-potsdam.de/frontdoor/index/index/docId/53444
Autor:
Erik Schliebs, Burkhard Micheel, Peter Schierack, Cindy Grieß, Dirk Reinhold, Pamela Holzlöhner, Christian Hentschel, Anja Schlör, Jonas Füner, Alexander Goihl, Monique Butze, Dirk Roggenbuck, Katja Hanack, Mandy Sowa, Martin Listek
Publikováno v:
New biotechnology. 45
Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified as a major autoantigenic target in Crohn’s disease patients. It was reported recently that a long (GP2a) and a short (GP2b) isoform of GP2 exist and that in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f81b85ca73458b238206c64155c3a825
https://pubmed.ncbi.nlm.nih.gov/29635104
https://pubmed.ncbi.nlm.nih.gov/29635104
Autor:
Pamela Holzlöhner, Katja Hanack
Publikováno v:
Journal of Visualized Experiments.
Monoclonal antibodies are universal binding molecules and are widely used in biomedicine and research. Nevertheless, the generation of these binding molecules is time-consuming and laborious due to the complicated handling and lack of alternatives. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09fb911c02c79b2586653c5d421ffe91
https://doi.org/10.3791/54832-v
https://doi.org/10.3791/54832-v
Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2
Autor:
Hans-Ulrich Schulz, Christian Hentschel, Pamela Holzlöhner, Alexander Goihl, Dirk Roggenbuck, Katja Hanack, Peter Schierack, Dirk Reinhold, Miklos Veiczi
Publikováno v:
Clinical chemistry and laboratory medicine. 55(6)
Background:Glycoprotein 2 (GP2), the pancreatic major zymogen granule membrane glycoprotein, was reported to be elevated in acute pancreatitis in animal models.Methods:Enzyme-linked immunosorbent assays (ELISAs) were developed to evaluate human glyco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d6dd8f92a391513c5deb2d9c3b84457
https://pubmed.ncbi.nlm.nih.gov/27837595
https://pubmed.ncbi.nlm.nih.gov/27837595
Publikováno v:
European Journal of Microbiology and Immunology. 2:231-238
Viral proteins are highly antigenic and known as potent stimulators of adaptive immune responses. This mechanism is often used for biotechnological applications in monoclonal antibody production resulting in high-affinity IgG antibodies in most cases
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1789f6eadc72e3de02b5d53eb81bf2bf
https://doi.org/10.1556/eujmi.2.2012.3.9
https://doi.org/10.1556/eujmi.2.2012.3.9
Publikováno v:
Journal of Biotechnology. 156:173-181
The production of monoclonal antibodies by hybridoma technology is dependent on lymphocytes taken from vertebrates which have to be immunized against the corresponding antigen. We present here our first experiments which should allow the replacement
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f998256292a1a0736b381ecfa88987ca
https://doi.org/10.1016/j.jbiotec.2011.09.002
https://doi.org/10.1016/j.jbiotec.2011.09.002
Autor:
Robert Sabat, Christian Sina, Kerstin Wolk, Elianne Tchatchou, Ute Hoffmann, Verena Moos, Katja Heilmann, Pamela Holzlöhner, Ursula Günthert, Stefan Schreiber, C Hayford, Martin Zeitz, Bianca M. Wittig, Ellen Witte, Christoph Loddenkemper
Publikováno v:
Journal of Cellular and Molecular Medicine
Osteopontin (OPN) is characterized as a major amplifier of Th1-immune responses. However, its role in intestinal inflammation is currently unknown. We found considerably raised OPN levels in blood of wild-type (WT) mice with dextran sodium sulfate (D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1a28372d3653e09842860707d3d445a
http://europepmc.org/articles/PMC4496111
http://europepmc.org/articles/PMC4496111
Autor:
Katja Hanack, Anja Schlör, Pamela Holzlöhner, Martin Listek, Cindy Bauer, Monique Butze, Burkhard Micheel, Christian Hentschel, Mandy Sowa, Dirk Roggenbuck, Peter Schierack, Jonas Füner, Erik Schliebs, Alexander Goihl, Dirk Reinhold
Publikováno v:
The Journal of Immunology. 196:209.8-209.8
Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn’s disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c112a12fce68cffdf64cc355bdcbd5ec
https://doi.org/10.4049/jimmunol.196.supp.209.8
https://doi.org/10.4049/jimmunol.196.supp.209.8
Autor:
Katja Heilmann, Pamela Holzlöhner
Publikováno v:
The Journal of Immunology. 194:206.14-206.14
Monoclonal antibodies are one of the most important tools in biomedicine but the generation by hybridoma technology is very time consuming and laborious. The adaptation of the process to an in vitro approach is therefore a smart alternative to reduce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::12d4a344418b0c25cdf1280f3820d906
https://doi.org/10.4049/jimmunol.194.supp.206.14
https://doi.org/10.4049/jimmunol.194.supp.206.14
Publikováno v:
The Journal of Immunology. 188:58.3-58.3
The production of monoclonal antibodies by hybridoma technology is dependent on lymphocytes taken from vertebrates which have to be immunized against the corresponding antigen. We present here our first experiments which should allow the replacement
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c6c7ae1d803f1764eb1da71211cf54fe
https://doi.org/10.4049/jimmunol.188.supp.58.3
https://doi.org/10.4049/jimmunol.188.supp.58.3