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pro vyhledávání: '"Pamela Herbert"'
Autor:
Ian Stansfield, Pamela Herbert, Kerrie M. Jones, William V. Shaw, Ann Lewendon, Mick F. Tuite
Publikováno v:
Journal of Molecular Biology. 282:13-24
We describe the development of a novel plasmid-based assay for measuring the in vivo frequency of misincorporation of amino acids into polypeptide chains in the yeast Saccharomyces cerevisiae. The assay is based upon the measurement of the catalytic
Publikováno v:
European Journal of Biochemistry. 131:313-324
The study of gel-filtered rabbit reticulocyte lysates and lysates which have been passed through 2′5′ ADP-Sepharose columns has shown that the presence of a sugar phosphate and a reducing system is necessary to maintain maximum rates of protein s
Publikováno v:
European Journal of Biochemistry. 131:303-311
Rabbit reticulocyte lysates were passed through 2′5′ ADP-Sepharose columns under conditions in which the gelfiltration effect was negligible and low-molecular-weight compounds were retained in the flow-through lysate. Glucose-6-phosphate dehydrog
Publikováno v:
European journal of biochemistry. 131(2)
When rabbit reticulocyte lysates were gel-filtered on Sephadex G-25 or G-50, the rate of protein synthesis in the gel-filtered lysate was the same as in the parent lysate provided appropriate concentrations of ATP, GTP and spermidine or spermine were