Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Pamela Hawley-Nelson"'
Autor:
Arshia Mathew, Pamela Hawley-Nelson, Thomas Albanetti, Lina Chakrabarti, Lina Li, Soo-Jin Han, Judith Klover
Publikováno v:
Journal of Immunological Methods. 464:31-39
Development of cell lines for biotherapeutic protein production requires screening large numbers of clones to identify and isolate high producing ones. As such, stable cell line generation is a time- and resource-intensive process. There is an increa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f72cbae5a82f4d1cef52d23174d851ff
https://doi.org/10.1016/j.jim.2018.10.007
https://doi.org/10.1016/j.jim.2018.10.007
Publikováno v:
Current Protocols in Molecular Biology
The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipid-based transfection reagents has accelerated biology research in studies of gene expression, control of cell grow
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1540b82f4327b441739d502ab03d9bb2
https://pubmed.ncbi.nlm.nih.gov/18428437
https://pubmed.ncbi.nlm.nih.gov/18428437
Publikováno v:
Current Protocols in Molecular Biology. 48
The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::460a1c659247bffe7eb6f2432c5bec31
https://doi.org/10.1002/0471142727.mb0904s48
https://doi.org/10.1002/0471142727.mb0904s48
Publikováno v:
Journal of Investigative Dermatology. 86(2):157-162
Skin calcium-binding protein (SCaBP) is a calcium binding protein purified from whole rat skin. It has a molecular weight of approximately 12,000 daltons but migrates at Mr 13,000 on sodium dodecyl sulfate (SDS)-polyacrylamide gels. On nitrocellulose
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80f5a63bd76a9ca9613825834eae019c
Publikováno v:
Molecular Carcinogenesis. 1:202-211
A gamma gt 11 cDNA expression library representing mouse epidermis mRNA was screened with polyclonal rabbit antiserum directed against 10-13 kDa epidermal antigens that had previously been shown to be regulated during epidermal differentiation. A cDN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cea2fbcb92a466af780a90e99679838
https://doi.org/10.1002/mc.2940010309
https://doi.org/10.1002/mc.2940010309
Publikováno v:
Journal of Investigative Dermatology. 75:183-186
In order to determine whether pemphigold antigen is synthesized by epidermal cells, and whether other, normal keratinocyte, antigens are synthesized in culture, primary cultures and subsequent subcultures of human epidermal cells derived from neonata
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34be8bfe795b4b2b48f0cd9aa85e5fc6
https://doi.org/10.1111/1523-1747.ep12522615
https://doi.org/10.1111/1523-1747.ep12522615
Publikováno v:
Journal of Investigative Dermatology. 81(1):S144-S149
Complementary DNA (cDNA) clones constructed to the 55, 59 and 67 kilodalton (K) keratins, the major keratins synthesized in newborn mouse epidermis, were used as molecular hybridization probes to examine the expression of these genes in newborn epide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d26fa2809ba0bb5ed507fd7be5a857f
Autor:
Pamela Hawley-Nelson, Beda M. Stadler, Gunther Grabner, John A. Schmidt, Joost J. Oppenheim, Bia M. Luger, Thomas A. Luger, Marcelo B. Sztein
Publikováno v:
Journal of Investigative Dermatology. 81(3):187-193
In this study we show that both cultured normal human epidermal cells (EC) and a human squamous cell carcinoma (SCC) cell line produce a thymocyte-activating factor (ETAF). EC-ETAF and SCC-ETAF both have a Mr of 15,000 and were eluted from chromatofo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::251852b2caddcb7058c0969c7cbf86c3
Publikováno v:
Journal of Investigative Dermatology. 87(4):454-459
Proteins from mouse epidermis cytosol extracts react on immunoblots with a polyclonal rabbit antiserum raised against rat skin calcium-binding protein (SCaBP), a parvalbumin of the panniculus carnosus. Three mouse epidermal proteins with molecular we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef1958a2ff7a3d12d6e066971824b0ff
Publikováno v:
The Journal of investigative dermatology. 75(2)
Methods have been optimized for the isolation, growth and passage of differentiating human epidermal cells in dispersed cell culture. Human neonatal foreskin epidermis was separated from dermis after floating the skin on trypsin. Keratinocytes were i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c816a383dd28aae7affbf6d93f8d9a9
https://pubmed.ncbi.nlm.nih.gov/6967921
https://pubmed.ncbi.nlm.nih.gov/6967921