Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Pamela A. Thuman-Commike"'
Publikováno v:
Virology. 411:393-415
The mosaic composition of the genomes of dsDNA tailed bacteriophages (Caudovirales) is well known. Observations of this mosaicism have generally come from comparisons of small numbers of often rather distantly related phages, and little is known abou
Publikováno v:
Biophysical Journal. 84(4):2585-2592
Bacteriophage assembly frequently begins with the formation of a precursor capsid that serves as a DNA packaging machine. The DNA packaging is accompanied by a morphogenesis of the small round precursor capsid into a large polyhedral DNA-containing m
Autor:
Pamela A Thuman-Commike
Publikováno v:
FEBS Letters. 505:199-205
Three-dimensional structure determination of macromolecules and macromolecular complexes is an integral part of understanding biological functions. For large protein and macromolecular complexes structure determination is often performed using electr
Autor:
Jonathan King, Zhixian Zhang, Barrie Greene, Pamela A. Thuman-Commike, Joanita Jakana, Wah Chiu, Peter E. Prevelige
Publikováno v:
Journal of Molecular Biology. 297:615-626
Large-scale conformational transitions are involved in the life-cycle of many types of virus. The dsDNA phages, herpesviruses, and adenoviruses must undergo a maturation transition in the course of DNA packaging to convert a scaffolding-containing pr
Autor:
Amy McGough, Justine A. Malinski, Wah Chiu, Barrie Greene, Peter E. Prevelige, Pamela A. Thuman-Commike, Michelle Burbea
Publikováno v:
Biophysical Journal. 76:3267-3277
Assembly of certain classes of bacterial and animal viruses requires the transient presence of molecules known as scaffolding proteins, which are essential for the assembly of the precursor procapsid. To assemble a procapsid of the proper size, each
Autor:
Pamela A. Thuman-Commike, Hiro Tsuruta, Wah Chiu, Peter E. Prevelige, Barrie Greene, Jonathan King
Publikováno v:
Biophysical Journal. 76(4):2249-2261
Structure factor amplitudes and phases can be computed directly from electron cryomicroscopy images. Inherent aberrations of the electromagnetic lenses and other instrumental factors affect the structure factors, however, resulting in decreased accur
Autor:
Joanita Jakana, Wah Chiu, Barrie Greene, Pamela A. Thuman-Commike, Peter E. Prevelige, Jonathan King, Venkataram B.V. Prasad
Publikováno v:
Journal of Molecular Biology. 260:85-98
The procapsids of bacterial viruses are the products of the polymerization of coat and scaffolding subunits, as well as the precursors in DNA packaging. Electron cryo-microscopy has been used to study the three-dimensional structures of bacteriophage
Autor:
Pamela A. Thuman-Commike, Wah Chiu
Publikováno v:
Microscopy and Microanalysis. 1:191-201
In this article we present a method to identify and extract spherical particle images from noisy spot-scan and flood beam electron microscopy images. We use a template matching algorithm with additional image preprocessing operations to allow consist
Autor:
Joanita Jakana, Barrie Greene, Amy McGough, Pamela A. Thuman-Commike, Peter E. Prevelige, Wah Chiu
Publikováno v:
Journal of virology. 74(8)
Critical stages in many viral life cycles involve large-scale conformational transitions. In certain classes of viruses, including the herpesviruses, adenoviruses, and double-stranded-DNA bacteriophages, the regulation of these transitions requires s