Zobrazeno 1 - 10
of 405
pro vyhledávání: '"Pamela A, Silver"'
Autor:
Samuel Lim, Charles B. Reilly, Zeina Barghouti, Benedetto Marelli, Jeffrey C. Way, Pamela A. Silver
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-10 (2024)
Abstract Tardigrades, microscopic animals that survive a broad range of environmental stresses, express a unique set of proteins termed tardigrade-specific intrinsically disordered proteins (TDPs). TDPs are often expressed at high levels in tardigrad
Externí odkaz:
https://doaj.org/article/4241eadef6b14aef831d0b2ddee62752
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-8 (2023)
Abstract In gene therapy, potential integration of therapeutic transgene into host cell genomes is a serious risk that can lead to insertional mutagenesis and tumorigenesis. Viral vectors are often used as the gene delivery vehicle, but they are pron
Externí odkaz:
https://doaj.org/article/fabe5d356c4b46a5b12156382aca5cc0
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Abstract Encapsulins are recently discovered protein compartments able to specifically encapsulate cargo proteins in vivo. Encapsulation is dependent on C-terminal targeting peptides (TPs). Here, we characterize and engineer TP-shell interactions in
Externí odkaz:
https://doaj.org/article/91c356a784e24cafbcf78672c4a1ab34
Autor:
Tai L. Ng, Erika J. Olson, Tae Yeon Yoo, H. Sloane Weiss, Yukiye Koide, Peter D. Koch, Nathan J. Rollins, Pia Mach, Tobias Meisinger, Trenton Bricken, Timothy Z. Chang, Colin Molloy, Jérôme Zürcher, Roger L. Chang, Timothy J. Mitchison, John I. Glass, Debora S. Marks, Jeffrey C. Way, Pamela A. Silver
Publikováno v:
mSystems, Vol 7, Iss 2 (2022)
ABSTRACT Suppression of the host innate immune response is a critical aspect of viral replication. Upon infection, viruses may introduce one or more proteins that inhibit key immune pathways, such as the type I interferon pathway. However, the abilit
Externí odkaz:
https://doaj.org/article/37ee1fa315624f72abfb551a9d58f267
Autor:
Bryan B. Hsu, Isaac N. Plant, Lorena Lyon, Frances M. Anastassacos, Jeffrey C. Way, Pamela A. Silver
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
It is difficult to precisely target bacterial populations in the mammalian gut. Here the authors use encapsulated phages to deliver dCas9 to E. coli in the mouse gut to modulate RFP expression.
Externí odkaz:
https://doaj.org/article/792e81c6c1904cf7ba9b6609a0a6e187
Autor:
Rolando Perez, Marina Luccioni, Rohinton Kamakaka, Samuel Clamons, Nathaniel Gaut, Finn Stirling, Katarzyna P. Adamala, Pamela A. Silver, Drew Endy
Publikováno v:
Fungal Biology and Biotechnology, Vol 7, Iss 1, Pp 1-15 (2020)
Abstract Background Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on single-cell organisms and extensive ancillary equipment to produce precursors for downstream manufacturing
Externí odkaz:
https://doaj.org/article/61a86c2e150545cdb1b6998629da9139
Autor:
David T. Riglar, David L. Richmond, Laurent Potvin-Trottier, Andrew A. Verdegaal, Alexander D. Naydich, Somenath Bakshi, Emanuele Leoncini, Lorena G. Lyon, Johan Paulsson, Pamela A. Silver
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Synthetic gene oscillators can be used to control timed function and periodic expression of genes. Here the authors demonstrate in vivo implementation in the mammalian gut that can keep time over several days.
Externí odkaz:
https://doaj.org/article/318e46930f814075b90ecd9d069da686
Autor:
Rui Tong Quek, Kierra S. Hardy, Stephen G. Walker, Dan T. Nguyen, Taciani de Almeida Magalhães, Adrian Salic, Sujatha M. Gopalakrishnan, Pamela A. Silver, Timothy J. Mitchison
Publikováno v:
ACS Chemical Biology. 18:583-594
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::47174a7a4a308856f7be52a23131c12a
https://doi.org/10.1021/acschembio.2c00908
https://doi.org/10.1021/acschembio.2c00908
Publikováno v:
mSystems, Vol 5, Iss 1 (2020)
ABSTRACT Elimination or alteration of select members of the gut microbiota is key to therapeutic efficacy. However, the complexity of these microbial inhabitants makes it challenging to precisely target bacteria. Here, we deliver exogenous genes to s
Externí odkaz:
https://doaj.org/article/8dbea1a9932446fc881019821f8b274f
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
Compartmentalization of proteins can potentially increase the productivity of engineered metabolic pathways. Here the authors use encapsulins to build non-endogenous organelles in Saccharomyces cerevisiae.
Externí odkaz:
https://doaj.org/article/8acf6e9857834d91a3d30674051ecf08