A Crude Extract Preparation and Optimization from a Genomically Engineered Escherichia coli for the Cell-Free Protein Synthesis System: Practical Laboratory Guideline.

Autor: Kim J; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA 70803, USA., Copeland CE; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA 70803, USA., Padumane SR; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA 70803, USA., Kwon YC; Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA 70803, USA. yckwon@lsu.edu.; Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA. yckwon@lsu.edu.

Publikováno v: Methods and protocols [Methods Protoc] 2019 Aug 09; Vol. 2 (3). Date of Electronic Publication: 2019 Aug 09.