Zobrazeno 1 - 10
of 53
pro vyhledávání: '"Padraig Doolan"'
Autor:
Noelia Sanchez, Mark Gallagher, Nga Lao, Clair Gallagher, Colin Clarke, Padraig Doolan, Sinead Aherne, Alfonso Blanco, Paula Meleady, Martin Clynes, Niall Barron
Publikováno v:
PLoS ONE, Vol 8, Iss 6, p e65671 (2013)
MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased leve
Externí odkaz:
https://doaj.org/article/3c7d73595041443e97e7b24bd5130b88
Publikováno v:
Biotechnology Letters. 42:927-936
To investigate the impact of polyamine deprivation on the transcriptome of CHO cells RESULTS: Polyamines play a central but poorly-understood role in cell proliferation. Most studies to date have utilised chemical inhibitors to probe polyamine functi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d5e7b90db350c0f9ce64d401ef25c70
https://doi.org/10.1007/s10529-020-02841-0
https://doi.org/10.1007/s10529-020-02841-0
Autor:
Fiona O'Neill, Martin Clynes, Finbarr O'Sullivan, Karina Horgan, Michael Henry, Joanne Keenan, Padraig Doolan, Paula Meleady, Richard Murphy, Niall Barron, Sinead T Aherne, Laura Breen, Colin Clarke
Publikováno v:
World Journal of Gastroenterology
AIM To identify miRNA-regulated proteins differentially expressed between Caco2 and HT-29: two principal cell line models of the intestine. METHODS Exponentially growing Caco-2 and HT-29 cells were harvested and prepared for mRNA, miRNA and proteomic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea39ecc2966b0c4000e4414f00956ac5
https://doi.org/10.3748/wjg.v23.i41.7369
https://doi.org/10.3748/wjg.v23.i41.7369
Publikováno v:
J Biol Chem
Polyamines have essential roles in cell proliferation, DNA replication, transcription, and translation processes, with intracellular depletion of putrescine, spermidine, and spermine resulting in cellular growth arrest and eventual death. Serum-free
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6be22203a690ad2cff03170c1e383d61
https://pubmed.ncbi.nlm.nih.gov/31666335
https://pubmed.ncbi.nlm.nih.gov/31666335
Publikováno v:
Biotechnology progressREFERENCES. 36(3)
While reliable transfection methods are essential for Chinese hamster ovary (CHO) cell line engineering, reduced transfection efficiencies have been observed in several commercially prepared media. In this study, we aimed to assess common media addit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7733dc0b9f6038507bfd3e3d00c1d49c
https://pubmed.ncbi.nlm.nih.gov/31850663
https://pubmed.ncbi.nlm.nih.gov/31850663
Autor:
Raphaela Ferreira, Thomas Glennon, Kevin Keating, Jens Ducrée, Padraig Doolan, Ricardo Valdes-Bango Currell, Damien King, Josh Fallon-Doran, Berta Capella Roca, Jonathan H. Loftus
Publikováno v:
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII).
Increased demands for protein production efficiency by identification of optimal protein producer cells and more stringent FDA requirements regarding single cell clonality represent key unmet needs within the biopharma industry. A microfluidic single
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9801a22ec12502791c9813fb79783e9c
https://doi.org/10.1109/transducers.2019.8808220
https://doi.org/10.1109/transducers.2019.8808220
Autor:
Padraig Doolan, Antonio Alarcon Miguez, Joanne Keenan, Srinivas Suda, Martin Clynes, Berta Capella Roca, Niall Barron, Donal J. O’Gorman
Publikováno v:
Cytotechnology
In order to study the impact of zinc and copper on the titer levels of mAb and recombinant protein in CHO cells, the IgG-expressing (DP12) and EPO-expressing (SK15) cell lines were cultured in chemically defined media with increasing concentrations o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::460bceaa612956c4e4dd524c9d2de345
https://pubmed.ncbi.nlm.nih.gov/31396753
https://pubmed.ncbi.nlm.nih.gov/31396753
Autor:
Maria Prencipe, Martin Clynes, Amanda O'Neill, Dara Lundon, R.W. Watson, Elaine W. Kay, G. Hurley, Sinead T Aherne, John M. Fitzpatrick, A. Boland, Colm Morrissey, Padraig Doolan, Stephen F. Madden
Publikováno v:
BMC Cancer
Background Docetaxel based therapy is one of the first line chemotherapeutic agents for the treatment of metastatic castrate-resistant prostate cancer. However, one of the major obstacles in the treatment of these patients is docetaxel-resistance. De
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4359c3206a6ae75bf2c985cf8caba036
http://europepmc.org/articles/PMC5333466
http://europepmc.org/articles/PMC5333466
Autor:
Colin Clarke, Laura Breen, Mark Leonard, Niall Barron, Sinead T Aherne, Paula Kinsella, Paula Meleady, Padraig Doolan, Martin Clynes, Lin Zhang
Publikováno v:
Journal of Biotechnology. 166:105-113
The selection of clones displaying a high rate of cell growth is an essential component of Chinese hamster ovary (CHO) cell line development. In recent years various "omics" technologies have been utilised to understand the mechanisms underlying biop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4401a82451ca09daa54e1131b3158817
https://doi.org/10.1016/j.jbiotec.2013.04.014
https://doi.org/10.1016/j.jbiotec.2013.04.014
Autor:
Maria Prencipe, William Watson, Amanda O'Neill, Sinead Ahearne, Dara Lundon, Stephen F. Madden, Padraig Doolan
Publikováno v:
Journal of Urology. 195
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1ed743af15c8c23822d9d05a25028571
https://doi.org/10.1016/j.juro.2016.02.2229
https://doi.org/10.1016/j.juro.2016.02.2229