Structure and function of H+/K+ pump mutants reveal Na+/K+ pump mechanisms

Autor: Victoria C. Young, Hanayo Nakanishi, Dylan J. Meyer, Tomohiro Nishizawa, Atsunori Oshima, Pablo Artigas, Kazuhiro Abe

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)

Here, the authors study ion transport mechanisms by introducing mutations to convert an electroneutral proton/potassium pump into a prototypical electrogenic sodium/potassium pump, explaining their selectivity and phosphorylation mechanisms.

Externí odkaz: https://doaj.org/article/1b8af5abb9244d9883fe5a760cf596d4

The selectivity of the Na+/K+-pump is controlled by binding site protonation and self-correcting occlusion

Autor: Huan Rui, Pablo Artigas, Benoît Roux

Publikováno v: eLife, Vol 5 (2016)

The Na+/K+-pump maintains the physiological K+ and Na+ electrochemical gradients across the cell membrane. It operates via an 'alternating-access' mechanism, making iterative transitions between inward-facing (E1) and outward-facing (E2) conformation

Externí odkaz: https://doaj.org/article/5e19ee2ce6484533b9e5acc610af408e

Cardiac Na/K-ATPase alpha1 isoform-specific role in hypertrophic response: role of metabolic and oxidative signaling

Autor: Marco Pessoa, Pauline Marck, Maddie Perdoncin, Dominic Collins, Liquan Cai, Jiang Tian, Pablo Artigas, Gustavo Blanco, Zijian Xie, Sandrine Pierre

Publikováno v: Physiology. 38

Cardiac Na/K-ATPase (NKA) inhibition by cardiotonic steroids (CTS) modulates intracellular Na+ and Ca2+, leading to increased force of contraction. In contrast, hypertrophy of cardiomyocytes (CM) is triggered by CTS through stimulation of reactive ox

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c25ad15583ffe306b31dfc810be00f81
https://doi.org/10.1152/physiol.2023.38.s1.5733247

Role of a conserved ion-binding site tyrosine in ion selectivity of the Na+/K+ pump

Autor: Kerri Spontarelli, Daniel T. Infield, Hang N. Nielsen, Rikke Holm, Victoria C. Young, Jason D. Galpin, Christopher A. Ahern, Bente Vilsen, Pablo Artigas

Publikováno v: Spontarelli, K, Infield, D T, Nielsen, H N, Holm, R, Young, V C, Galpin, J D, Ahern, C A, Vilsen, B & Artigas, P 2022, ' Role of a conserved ion-binding site tyrosine in ion selectivity of the Na + /K + pump ', Journal of General Physiology, vol. 154, no. 7, e202113039 . https://doi.org/10.1085/jgp.202113039
Spontarelli, K, Infield, D T, Nielsen, H N, Holm, R, Young, V C, Galpin, J D, Ahern, C A, Vilsen, B & Artigas, P 2022, ' Role of a conserved ion-binding site tyrosine in ion selectivity of the Na/K pump ', pp. 27 . < https://www.cell.com/biophysj/pdf/S0006-3495(21)03555-4.pdf >

The Na/K pump hydrolyses ATP to export three Na and import two Kacross the plasmalemma. Crystal structures identified three ion-binding siteswithin the pump’s a-subunit. Site-I and site-II bind either Na or K, whilesite-III exclusively binds Na. A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3eef491355124e8ee3bbb378878c337a
https://pure.au.dk/portal/da/publications/role-of-a-conserved-ionbinding-site-tyrosine-in-ion-selectivity-of-the-nak-pump(bfb93d49-7234-4b6b-bbca-43b48d5d24fd).html

Functional analysis of plasma membrane Ca2+ATPase 3 and its primary aldosteronism-associated mutations

Autor: Victoria C. Young, Marilina de Sautu, Irene C. Mangialavori, Pablo Artigas

Publikováno v: Biophysical Journal. 122:528a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6523395439eabe20d987c1c7afb0f169
https://doi.org/10.1016/j.bpj.2022.11.2804