Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases

Autor: Erin E. Drufva, Constance B. Bailey, Elijah G. Hix

Publikováno v: Synthetic and Systems Biotechnology, Vol 5, Iss 2, Pp 62-80 (2020)
Synthetic and Systems Biotechnology

Modular polyketide synthases (PKSs) are a multidomain megasynthase class of biosynthetic enzymes that have great promise for the development of new compounds, from new pharmaceuticals to high value commodity and specialty chemicals. Their colinear bi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b84c5447c1f65c695a30aae495cef9d
https://doi.org/10.1016/j.synbio.2020.04.001

Engineered polyketides: Synergy between protein and host level engineering

Autor: Constance B. Bailey, Jesus F. Barajas, Samuel C. Curran, Jacquelyn M. Blake-Hedges, Jay D. Keasling

Publikováno v: Synthetic and Systems Biotechnology, Vol 2, Iss 3, Pp 147-166 (2017)
Synthetic and Systems Biotechnology
Synthetic and systems biotechnology, vol 2, iss 3
Barajas, J F, Blake-Hedges, J M, Bailey, C B, Curran, S & Keasling, J D 2017, ' Engineered polyketides: Synergy between protein and host level engineering ', Synthetic and Systems Biotechnology, vol. 2, no. 3, pp. 147-166 . https://doi.org/10.1016/j.synbio.2017.08.005
Barajas, JF; Blake-Hedges, JM; Bailey, CB; Curran, S; & Keasling, JD. (2017). Engineered polyketides: Synergy between protein and host level engineering. Synthetic and Systems Biotechnology, 2(3), 147-166. doi: 10.1016/j.synbio.2017.08.005. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/6908v16f

© 2017 Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities. Polyketides enc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::434d695113b544867aef42aadbfb2c86
https://doi.org/10.1016/j.synbio.2017.08.005

Informatic analysis reveals

Autor: Chad W, Johnston, Jonathan, Plumb, Xiang, Li, Sergio, Grinstein, Nathan A, Magarvey

Publikováno v: Synthetic and Systems Biotechnology

Microbial natural products are a crucial source of bioactive molecules and unique chemical scaffolds. Despite their importance, rediscovery of known natural products from established productive microbes has led to declining interest, even while emerg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a605666b7e0532486f97d6b7b0ca0235
https://pubmed.ncbi.nlm.nih.gov/29062936

Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets.

Autor: Mohite OS; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark., Lloyd CJ; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA., Monk JM; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA., Weber T; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark., Palsson BO; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA.

Publikováno v: Synthetic and systems biotechnology [Synth Syst Biotechnol] 2022 May 06; Vol. 7 (3), pp. 900-910. Date of Electronic Publication: 2022 May 06 (Print Publication: 2022).

Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases.

Autor: Drufva EE; Department of Chemistry, University of Tennessee-Knoxville, Knoxville, TN, 37996, USA., Hix EG; Department of Chemistry, University of Tennessee-Knoxville, Knoxville, TN, 37996, USA., Bailey CB; Department of Chemistry, University of Tennessee-Knoxville, Knoxville, TN, 37996, USA.

Publikováno v: Synthetic and systems biotechnology [Synth Syst Biotechnol] 2020 May 13; Vol. 5 (2), pp. 62-80. Date of Electronic Publication: 2020 May 13 (Print Publication: 2020).