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Identification and characterization of a family of secretion-related small GTPase-encoding genes from the filamentous fungus Aspergillus niger : a putative SEC4 homologue is not essential for growth

Autor: C.A.M.J.J. van den Hondel, C. Konetschny, A.F.J. (Arthur) Ram, C. M. J. Van Zeijl, P.J. Punt, Bernhard Seiboth, M. Lenders, Roy Christiaan Montijn, X.O. Weenink, Christian P. Kubicek

Publikováno v: Molecular Microbiology. 41:513-525

DNA fragments containing genetic information for five secretion-related small GTPases of Aspergillus niger (srgA-E) were isolated and identified as members of different Rab/Ypt subfamilies. This isolation and the search for similar sequences in funga

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3b712eabe74898138be1e6ad014d2043
https://doi.org/10.1046/j.1365-2958.2001.02541.x

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GlaA Promoter Controlled Production of a Mutant Green Fluorescent Protein (S65T) by Recombinant Aspergillus niger during Growth on Defined Medium in Batch and Fed-Batch Cultures

Autor: D. Siedenberg, Ursula Rinas, Mestric Silvija, P.J. Punt, Michael Schmidt, Markus Ganzlin, C.A.M.J.J. van den Hondel

Publikováno v: Biotechnology Progress. 15:43-50

The first successful expression of the Aequorea victoria green fluorescent protein (GFP) gene in Aspergillus niger is described. When the wild-type GFP gene was expressed in A. niger, neither the fluorescence nor the full translation product of the w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c271d0050211976526835ad61cc1e497
https://doi.org/10.1021/bp980105u

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Analysis of the role of the gene bipA , encoding the major endoplasmic reticulum chaperone protein in the secretion of homologous and heterologous proteins in black Aspergilli

Autor: J. Drint-Kuijvenhoven, C.A.M.J.J. van den Hondel, A. Beijersbergen, P.J. Punt, J. G. M. Hessing, Cornelis Theodorus Verrips, G. M. van Muijlwijk-Harteveld, I. A. van Gemeren

Publikováno v: Applied Microbiology and Biotechnology, 4, 50, 447-454

The function of the endoplasmic-reticulum-localized chaperone binding protein (BiP) in relation to protein secretion in filamentous fungi was studied. It was shown that the overproduction of several homologous and heterologous recombinant proteins by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec68e60264ce220006a416bf6ce49b9b
https://doi.org/10.1007/s002530051319

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Optimization and stability of glucoamylase production by recombinant strains ofAspergillus niger in chemostat culture

Autor: Julie M. Withers, Richard J. Swift, Geoffrey D. Robson, Marilyn G. Wiebe, P.J. Punt, C. A. M. J. J. Van Den Hondel, Anthony P. J. Trinci

Publikováno v: Biotechnology and Bioengineering. 59:407-418

When grown on a medium containing 5 g maltodextrin L-1, Aspergillus niger transformant N402[pAB6-10]B1, which has an additional 20 copies of the glucoamylase (glaA) gene, produced 320 +/- 8 mg (mean +/- S.E.) glucoamylase (GAM) L-1 in batch culture a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1eca0e1084ea5c4e80ae74ca418ec07f
https://doi.org/10.1002/(sici)1097-0290(19980820)59:4<407::aid-bit3>3.0.co

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Kinetics of mRNA and protein synthesis of genes controlled by the 1,4-beta-endoxylanase A promoter in controlled fermentations of Aspergillus awamori

Autor: A.J. Fellinger, R. J. Gouka, Wouter Musters, P.J. Punt, C.A.M.J.J. van den Hondel, Hein Stam, A.J. van de Wijngaard, R.J.G.T. Muijsenberg

Publikováno v: Applied and Environmental Microbiology. 62:3646-3649

In this study, induction and repression kinetics of the expression of the Aspergillus awamori 1,4-beta-endoxylanase A (exlA) gene under defined physiological conditions was analyzed at the mRNA and the protein levels. Induction was analyzed by pulsin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c697b04afea5d785bbcadd79ea742a3
https://doi.org/10.1128/aem.62.10.3646-3649.1996

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A mini-promoter lacZ gene fusion for the analysis of fungal transcription control sequences

Autor: C.A.M.J.J. van den Hondel, P.J. Punt, Anneke Kuyvenhoven

Publikováno v: Gene. 158:119-123

A system for the in vivo analysis of fungal transcription control sequences, based on a mini-promoter, was designed. The mini-promoter, providing all sequences necessary and sufficient for transcription initiation, was derived from the Aspergillus ni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6de7a2920fc9e52cdf17effa7194d580
https://doi.org/10.1016/0378-1119(95)00166-4

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