Zobrazeno 1 - 10
of 192
pro vyhledávání: '"P.F. Greenfield"'
Publikováno v:
Proceedings of the Water Environment Federation. 2001:371-387
Autor:
P.F. Greenfield, Ziqi Liao
Publikováno v:
IEEE Transactions on Engineering Management. 45:323-330
This paper presents an empirical study on the strategic structure of corporate R&D in Japanese and Australian technology-based companies. The perceived importance and distribution of basic research, precompetitive strategic research and applied resea
Publikováno v:
Biotechnology Techniques. 6:393-398
A simple method using non-linear regression is developed to analyse experimental data from plasmid stability studies of recombinant Saccharomyces cerevisiae grown in continuous cultures with non-selective and selective media. This method simultaneous
Publikováno v:
Journal of Biotechnology. 14:311-320
The stability of a yeast plasmid can be improved using deliberately induced cyclic changes in the dissolved oxygen tension (DOT), during continuous culture in a non-selective, undefined medium. The resultant stability of the plasmid under DOT cycled
Autor:
P.F. Greenfield, L.K. Nielsen
On-line measurement of OUR using off-gas analysis equipment has been used to implement monitoring and advanced control schemes in several microbial fermentation systems. The development of similar applications for cell culture systems has been limite
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d5bae7c271d09cab4c13f61fac623731
https://doi.org/10.1016/b978-0-7506-1845-8.50094-6
https://doi.org/10.1016/b978-0-7506-1845-8.50094-6
Publikováno v:
Journal of Fermentation Technology. 65:449-456
The initial adsorption of a mixed bacterial culture, capable of degrading a phenolic wastestream, on a range of possible support particles was studied in a fluidized bed column. Activated carbon was found to be the most effective support in promoting
Publikováno v:
Journal of Biotechnology. 10:171-180
The stability of a 2μm based plasmid, pLG669-z, was measured in a Saccharomyces cerevisiae strain, grown continuously in a non-selective medium. The apparent stability of the plasmid was found to decrease with increasing growth rate. A model was dev
Publikováno v:
Biotechnology Letters. 11:605-608
The 2μm based yeast plasmid, pLG669-z, was stably maintained in a S. cerevisiae strain during continuous culture with cyclic growth rate changes. In the absence of a selective mechanism, the fraction of plasmid containing cells remained high, and ev
Publikováno v:
Journal of Biotechnology. 8:173-192
Yeasts represent one class of host for the production of recombinant proteins. Heterologous DNA is usually introduced into yeast strains in the form of multi-copy plasmids. During production, protein expression levels and rates are often limited by t