Otoferlin gene editing in sheep via CRISPR-assisted ssODN-mediated Homology Directed Repair

Autor: Laurent Tesson, Vanessa Chenouard, Martina Crispo, Ignacio Anegon, F. Cuadro, Jean-Marie Heslan, P.C. dos Santos-Neto, Alejo Menchaca, M Gantier, A. P. Mulet, Aude Guiffes, M Souza-Neves

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.5995. ⟨10.1038/s41598-020-62879-y⟩
Scientific Reports, 2020, 10 (1), pp.5995. ⟨10.1038/s41598-020-62879-y⟩
Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)

Different mutations of the OTOF gene, encoding for otoferlin protein expressed in the cochlear inner hair cells, induces a form of deafness that is the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. We report t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d09371df7112f3e31332a06b9d68e89c
https://www.hal.inserm.fr/inserm-02561070/document

Embryo survival and birth rate after minimum volume vitrification or slow freezing of in vivo and in vitro produced ovine embryos

Autor: P.C. dos Santos-Neto, Alejo Menchaca, Martina Crispo, N. Barrera, F. Cuadro

Publikováno v: Cryobiology. 78:8-14

The objective was to evaluate pregnancy outcomes and birth rate of in vivo derived vs. in vitro produced ovine embryos submitted to different cryopreservation methods. A total of 197 in vivo and 240 in vitro produced embryos were cryopreserved either

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e51707d167add40969ac2379a4adc798
https://doi.org/10.1016/j.cryobiol.2017.08.002

Cumulus cells during in vitro fertilization and oocyte vitrification in sheep: Remove, maintain or add?

Autor: M. Vilariño, N. Barrera, P.C. dos Santos-Neto, M. Crispo, F. Cuadro, Alejo Menchaca

Publikováno v: Cryobiology. 92

The objective was to evaluate the developmental competence of immature and matured ovine oocytes after removing, maintaining or adding cumulus cells (CC) associated to vitrification by Cryotop method. Three experiments were performed involving 3,144

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34f7eb5b22d44fcdb077483d5c3f8219
https://pubmed.ncbi.nlm.nih.gov/31917962

Fertility obtained with different progestogen intravaginal devices using Short-term protocol for fixed-time artificial insemination (FTAI) in sheep

Autor: C. García-Pintos, A. Pinczak, P.C. dos Santos-Neto, Alejo Menchaca

Publikováno v: Livestock Science. 182:125-128

The objective of this study was to evaluate the pregnancy outcomes obtained with either cervical or intrauterine insemination after Short-term protocols (6 days) for FTAI comparing different progestogens and intravaginal devices in sheep. Two experim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2a6a6381bd197db04abb08d0e8f2c273
https://doi.org/10.1016/j.livsci.2015.11.005

Oocyte developmental competence is improved by relatively greater circulating progesterone concentrations during preovulatory follicular growth

Autor: Alejo Menchaca, D. Bosolasco, Martina Crispo, P.C. dos Santos-Neto, F. Cuadro, N. Barrera, V. de Brun

Publikováno v: Animal reproduction science. 195

This study evaluated the effect of progesterone priming during follicular growth on oocyte competence to undergo oocyte cleavage and embryo development in sheep. Two experiments were performed on a total of 195 females that either received or did not

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a08b3e811795755244c5044dd57e7c9a
https://pubmed.ncbi.nlm.nih.gov/31262405