Zobrazeno 1 - 10
of 34
pro vyhledávání: '"P. T. Iype"'
Publikováno v:
Cytotechnology. 26:207-218
New human breast cell lines were developed from metastatic breast cancer tissues and normal breast tissues. Primary cultures were initiated from cellular outgrowths of explanted tissues or from mechanically isolated cells in two serum-free media. Cel
Publikováno v:
International Journal of Oncology.
New human prostate cell lines were developed from prostatic carcinoma (BRF-41T) and BPH (BRF-55T). Primary cultures were initiated from cellular outgrowths of explanted tissues. A serum-free medium, BRFF-HPC1, was developed for growing human prostati
Autor:
Janet M. Carter, H. L. Kumari, Robert J. Isfort, J. L. Burns, Leslie L. Crosby, Kevin E. Driscoll, Robert A. LeBoeuf, M. J. Aardema, David B. Cody, P. T. Iype, M. H. Chestnut
Publikováno v:
In vitro cellulardevelopmental biology. Animal. 31(7)
We developed methodology to isolate and culture rat alveolar Type II cells under conditions that preserved their proliferative capacity, and applied lipofection to introduce an immortalizing gene into the cells. Briefly, the alveolar Type II cells we
Publikováno v:
Mutation research. 248(1)
Monitoring human populations for specific DNA modifications has been made possible by developing highly sensitive immunoassays employing antibodies specific for carcinogen-DNA adducts. While these techniques have been used to follow occupationally an
Publikováno v:
Proceedings of the National Academy of Sciences. 79:456-460
An inhibitor of cell proliferation was purified from rat liver by alcohol precipitation, ultrafiltration, and DEAE-cellulose chromatography. The hepatic proliferation inhibitor was shown to be pure by polyacrylamide gel electrophoresis in the presenc
Publikováno v:
Journal of Biological Chemistry. 259:1803-1806
The subcellular and intralobular distributions of a protein which specifically inhibits the proliferation of normal liver cells were determined in rat liver, using a combination of immunological and biochemical techniques. The IgG fraction from an an
Publikováno v:
In Vitro. 12:837-844
A direct comparison has been made between normal parenchymal cells cultured from rat liver and malignant cells from both a rat liver tumor and a spontaneously transformed line derived from the parent rat culture. In all nondividing cells there was a
Publikováno v:
British Journal of Cancer
The effect of the tumour promoters TPA, phenobarbitone and saccharin on the production of sister-chromatid exchanges (SCE) was studied. TPA produced a small but significant increase of SCE in Chinese hamster cell lines V-79 and CHO, and in a hybrid c
Autor:
J. B. McMahon, P. T. Iype
Publikováno v:
Journal of Liquid Chromatography. 5:751-756
An improved method for the purification of hepatic proliferation inhibitor from rat liver by means of anion-exchange HPLC has been developed. The inhibitor can be purified on an anion exchange HPLC column by using a linear sodium phosphate gradient.
Publikováno v:
British Journal of Cancer
The occurrence of organ-specific antigens on the surface of cells freshly isolated from normal adult rat liver and from liver cell lines cultured as monolayers for up to 6 months is demonstrated. Enzyme treatment used to disaggregate parenchymal cell