Zobrazeno 1 - 10
of 21
pro vyhledávání: '"P. R. ROSEVEAR"'
Publikováno v:
Journal of biomolecular NMR. 17(1)
Several HNCO-based three-dimensional experiments are described for the measurement of 13C'(i - 1)-13Calpha(i - 1), 5N(i)-13C'(i - 1), 15N(i)-13Calpha(i), 15N(i)-13Calpha(i - 1), 1H(N)(i)-13Calpha(i), 1H(N)(i)-13Calpha(i - 1), and 13Calpha(i - 1)-13Cb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a7f3e52f4b56ce12c031b7b183548199
https://pubmed.ncbi.nlm.nih.gov/10909865
https://pubmed.ncbi.nlm.nih.gov/10909865
Publikováno v:
The Journal of biological chemistry. 269(38)
NMR spectroscopy and selective isotope labeling of both recombinant cardiac troponin C (cTnC3) and a truncated cardiac troponin I (cTnI/NH2) lacking the N-terminal 32-amino acid cardiac-specific sequence have been used to probe protein-protein intera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d139ca341a13e1e66de277b1c2bb514a
https://pubmed.ncbi.nlm.nih.gov/8089144
https://pubmed.ncbi.nlm.nih.gov/8089144
Publikováno v:
The Journal of biological chemistry. 268(28)
One- and two-dimensional NMR techniques were used to compare the structural consequences of Ca2+ binding to both the low and high affinity Ca2+ binding sites in recombinant cardiac troponin C (cTnC3). In the absence of Ca2+, the short beta-sheet loca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::25595b41b2832cfa559b70e7fdce1e42
https://pubmed.ncbi.nlm.nih.gov/8407932
https://pubmed.ncbi.nlm.nih.gov/8407932
Publikováno v:
The Journal of biological chemistry. 268(22)
A truncated form of the methionyl-tRNA synthetase (delta MTS), which has been cloned, overproduced, and characterized, was used in an attempt to better understand the role of the enzyme-bound zinc in the amino-acylation process. Apo-, Zn(2+)-, Co(2+)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6382695550fc0dd521eaadc5b1c9996d
https://pubmed.ncbi.nlm.nih.gov/8344912
https://pubmed.ncbi.nlm.nih.gov/8344912
Autor:
J S, Williams, P R, Rosevear
Publikováno v:
The Journal of biological chemistry. 266(4)
Internuclear distances obtained from nuclear Overhauser effects were used in combination with a distance geometry algorithm to determine the conformation of Mg(alpha,beta-methylene)ATP bound to the Escherichia coli truncated methionyl-tRNA synthetase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3830e553a4452343e3247c989856a72a
https://pubmed.ncbi.nlm.nih.gov/1989972
https://pubmed.ncbi.nlm.nih.gov/1989972
Publikováno v:
The Journal of biological chemistry. 265(2)
When a derivatized oligosaccharide isolated from ovalbumin and containing 6 mannose residues was incubated with yeast membranes and GDP-mannose, two sets of products were obtained, a high molecular weight one containing about 25 mannose residues and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f51fd6610f08c5b53b93406b5bd0ae6a
https://pubmed.ncbi.nlm.nih.gov/2404010
https://pubmed.ncbi.nlm.nih.gov/2404010
Autor:
P R, Rosevear
Publikováno v:
Biochemistry. 27(20)
A procedure for the rapid purification of a truncated form of the Escherichia coli methionyl-tRNA synthetase has been developed. With this procedure, final yields of approximately 3 mg of truncated methionyl-tRNA synthetase per gram of cells, carryin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9affe2a83f5b7e7559bee3849b18f927
https://pubmed.ncbi.nlm.nih.gov/3061464
https://pubmed.ncbi.nlm.nih.gov/3061464
Publikováno v:
Federation proceedings. 43(11)
NMR has been used to study the role of the divalent cation, the conformations, arrangement, and exchange rates of the enzyme-bound metal-ATP and peptide substrates, the mechanism of the phosphoryl transfer, and the structure and role of the regulator
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::56fa809a7774ebb6bfbb750a1cc1529c
https://pubmed.ncbi.nlm.nih.gov/6086412
https://pubmed.ncbi.nlm.nih.gov/6086412
Publikováno v:
The Journal of biological chemistry. 258(11)
The paramagnetic effects of Mn2+ . glyoxalase I on the 13C relaxation rates of the reaction product, S-(D-lactoyl)glutathione, separately enriched in the lactoyl carbonyl (C-1) and hydroxymethylene (C-2) carbons, have been measured at 62.8 MHz. The 1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1821a27672f441c1b66853414067f036
https://pubmed.ncbi.nlm.nih.gov/6853506
https://pubmed.ncbi.nlm.nih.gov/6853506
Publikováno v:
The Journal of biological chemistry. 259(18)
The conformations of four derivatives of glutathione bound at the active site of the metalloenzyme glyoxalase I have been determined by NMR measurements and by computer model building using a distance geometry approach. Paramagnetic effects of Mn2+-g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::44a655becc9e82c2f71b9f3025bb0317
https://pubmed.ncbi.nlm.nih.gov/6547959
https://pubmed.ncbi.nlm.nih.gov/6547959