Zobrazeno 1 - 10 of 24 pro vyhledávání: '"P. R. Housley"'
2
Optimization of Calcium Phosphate Transfection for Bovine Chromaffin Cells: Relationship to Calcium Phosphate Precipitate Formation
Autor: R. E. Wilson, P. R. Housley, Fang Liu, Steven P. Wilson
Publikováno v: Analytical Biochemistry. 226:212-220
Optimal conditions for formation of calcium phosphate-DNA precipitates and for chromaffin cell transfection by the calcium phosphate method were examined. A relationship was observed between turbidity of calcium phosphate solutions and the ability of
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::535e68bc50f0b41723c8721255823113
https://doi.org/10.1006/abio.1995.1216
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3
Potentiation of glucocorticoid receptor-mediated gene expression by heat and chemical shock
Autor: Edwin R. Sanchez, P. R. Housley, Suju Zhong, Michael J. Greene, Jun-Lian Hu, Ping Shen
Publikováno v: Molecular Endocrinology. 8:408-421
We have examined the effects of heat shock on glucocorticoid receptor (GR)-mediated gene transcription in an L929 cell line derivative (LMCAT2) stably transfected with the mouse mammary tumor virus-chloramphenicol acetyltransferase (MMTV-CAT) reporte
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::655f0be384089877ab092fb256f48e7a
https://doi.org/10.1210/mend.8.4.8052262
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5
Site-directed mutagenesis of the phosphorylation sites in the mouse glucocorticoid receptor
Autor: S A, Mason, P R, Housley
Publikováno v: The Journal of biological chemistry. 268(29)
The functional significance of receptor phosphorylation in mediating the actions of glucocorticoids remains undefined. The identification of seven phosphorylation sites in the mouse glucocorticoid receptor (Bodwell, J. E., Orti, E., Coull, J. M., Pap
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::10a3e7d041f9adde191cebb0eba62dbf
https://pubmed.ncbi.nlm.nih.gov/8407999
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6
Evidence that the conserved region in the steroid binding domain of the glucocorticoid receptor is required for both optimal binding of hsp90 and protection from proteolytic cleavage. A two-site model for hsp90 binding to the steroid binding domain
Autor: P R, Housley, E R, Sanchez, M, Danielsen, G M, Ringold, W B, Pratt
Publikováno v: The Journal of biological chemistry. 265(22)
Steroid hormone receptors contain a conserved sequence of amino acids within the steroid binding domain, and we have previously speculated that this conserved region is the site of interaction of the glucocorticoid receptor with hsp90 (Danielsen, M.,
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9334ebd889dee73af83502e8cca746b6
https://pubmed.ncbi.nlm.nih.gov/2376573
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7
Direct demonstration of glucocorticoid receptor phosphorylation by intact L-cells
Autor: W B Pratt, P R Housley
Publikováno v: Journal of Biological Chemistry. 258:4630-4635
Glucocorticoid-sensitive L-cells were cultured for 18 h in the presence of [32P]orthophosphate and steroid-binding proteins of cytosol were separated by affinity chromatography and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::41d26863822ff795848b0ad2af1a87ce
https://doi.org/10.1016/s0021-9258(18)32670-x
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8
Inactivation of glucocorticoid-binding capacity by protein phosphatases in the presence of molybdate and complete reactivation of dithiothreitol
Autor: William B. Pratt, P R Housley, Mary K. Dahmer
Publikováno v: Journal of Biological Chemistry. 257:8615-8618
Glucocorticoid receptor in rat liver cytosol is inactivated (rendered unable to bind steroid) by incubation with calf intestine alkaline phosphatase or highly purified rabbit muscle phosphoprotein phosphatase (phosphorylase phosphate, protein phospha
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::33b65a9963a74d6a6cf7e9fc34a01285
https://doi.org/10.1016/s0021-9258(18)34170-x
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9
Glucocorticoid receptor phosphorylation, transformation, and DNA binding
Autor: Edwin R. Sanchez, William B. Pratt, P R Housley, W Tienrungroj, R W Harrison
Publikováno v: Journal of Biological Chemistry. 262:17342-17349
Glucocorticoid receptors were isolated by immunoadsorption from cytosol of L cells that were cultured for 18 h in the presence of [32P]orthophosphate, and the phosphorylation state of the receptor was examined before and after transformation to the D
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::69cd0da97c924ccb4c959daf56538b84
https://doi.org/10.1016/s0021-9258(18)45383-5
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10
The molybdate-stabilized L-cell glucocorticoid receptor isolated by affinity chromatography or with a monoclonal antibody is associated with a 90-92-kDa nonsteroid-binding phosphoprotein
Autor: Miguel Beato, Edwin R. Sanchez, William B. Pratt, H M Westphal, P R Housley
Publikováno v: Journal of Biological Chemistry. 260:13810-13817
We have previously reported that molybdate-stabilized cytosol prepared from 32P-labeled L-cells contains two phosphoproteins (a 90-92- and a 98-100-kDa protein) that elute from an affinity resin of deoxycorticosterone-derivatized agarose in a manner
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8085c0ed8c46451f39b92657d5a78c1f
https://doi.org/10.1016/s0021-9258(17)38797-5
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