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pro vyhledávání: '"P. N. Asare Okai"'
Publikováno v:
Chemical Communications. 55:10456-10459
A bio-orthogonal chemistry-based approach for fluorescent labelling of ribosomal RNA is described. It involves an adenosine analogue modified with trans-cyclooctene and masked 5'-phosphate group using aryl phosphoramidate. The incorporation into rRNA
Autor:
P. N. Asare Okai, Irfan Khan, Michael R. Miller, Edgar Agustin, Jia Sheng, R. Wang, Maksim Royzen
Publikováno v:
Chemical Communications. 52:1405-1408
A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels-Alder reaction between trans-cyclooctene a