Zobrazeno 1 - 10
of 29
pro vyhledávání: '"P W, Melera"'
Publikováno v:
Journal of Biological Chemistry. 269:7976-7981
Resistance to the intracellular Ca2+ pump inhibitor thapsigargin (TG) is associated with overexpression of both Ca2+ transport ATPase and the multidrug resistance (mdr) transporter P-glycoprotein (pgp). This is supported by increased resistance to TG
Publikováno v:
Journal of cellular biochemistry. 81(2)
DC-3F/FA3 cells (FA3) were obtained by selection of Chinese hamster lung fibroblasts for growth in folic acid free media, supplemented with 15 pM [6S]-5-formyltetrahydrofolic acid. These cells, as a result of low level gene amplification and RNA stab
Autor:
W Y, Zhu, P W, Melera
Publikováno v:
Cancer research. 59(17)
DC-3F/FA3 (FA3) cells, selected for growth in folic acid-free medium containing dialyzed serum and 15 pM [6S]-folinic acid, and parental DC-3F cells were compared by mRNA differential display to identify genetic changes occurring during selection. On
Autor:
S E, Devine, P W, Melera
Publikováno v:
The Journal of biological chemistry. 269(8)
Mammalian cells displaying the multidrug resistance (mdr) phenotype are refractory to the toxic effects of a group of unrelated natural product drugs, many of which are used for cancer chemotherapy. The pattern of cross-resistance can be extremely va
Autor:
M, Yu, P W, Melera
Publikováno v:
Cancer research. 53(24)
The Chinese hamster lung cell line DC-3F contains two polymorphic dihydrofolate reductase (DHFR) alleles that are defined by an Asp-Asn amino acid sequence difference at position 95 in protein. Previously, we reported that the antifolate-resistant su
Autor:
H, Yang, P W, Melera
Publikováno v:
BioTechniques. 13(6)
We have developed a modified RNase protection assay in which the antisense RNA probe is prepared from a PCR-amplified DNA template rather than from a linearized plasmid DNA template. In this assay, an RNA polymerase promoter sequence is attached to t
Autor:
P W, Melera
Publikováno v:
Seminars in cancer biology. 2(4)
The expression of resistance by tumor cells to anticancer drugs remains a primary cause of patient failure to chemotherapeutic intervention in cancer. Although studied for many years, both the acquisition and maintenance of drug resistance continue t
Publikováno v:
The Journal of biological chemistry. 266(7)
In an effort to better understand the preferential resistance to actinomycin D displayed by the multidrug-resistant Chinese hamster lung cell line DC-3F/ADX, we have cloned from those cells a number of cDNAs representing p-glycoprotein gene transcrip
Publikováno v:
Cancer Genetics and Cytogenetics. 2:47-60
Specific karyotype alterations are described which occur in 16 different antifolate-resistant Chinese hamster sublines with increases in target enzyme dihydrofolate reductase activity ranging from 2.9- to 281-fold. Sublines were selected with either
Publikováno v:
Journal of Biological Chemistry. 255:319-322
A comparison by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins synthesized by antifolate-sensitive (DC-3F) and -resistant (DC-3F/A3 and DC-3F/MQ19) Chinese hamster lung fibroblast cells has shown that an apparent molecular