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Publikováno v:
Pflügers Archiv European Journal of Physiology. 431:868-875
Autor:
A K M, Hammarström, P W, Gage
Publikováno v:
Methods in enzymology. 381
Publikováno v:
British journal of pharmacology. 131(4)
Spontaneously opening, chloride-selective channels that showed outward rectification were recorded in ripped-off patches from rat cultured hippocampal neurons and in cell-attached patches from rat hippocampal CA1 pyramidal neurons in slices. In both
Publikováno v:
Molecular pharmacology. 58(3)
We examined the effect of a range of pentobarbital concentrations on 0.5 microM gamma-aminobutyric acid (GABA)-activated channels (10 +/- 1 pS) in inside-out or outside-out patches from rat cultured hippocampal neurons. The conductance increased from
Publikováno v:
Synapse (New York, N.Y.). 26(3)
The effects on the functional properties of the alpha 1 beta 1 GABAA receptor when the 5' (alpha 1 Val260; beta 1 Ile255) hydrophobic amino acids in the second transmembrane (M2) region were changed to threonine were examined. In response to a satura
Publikováno v:
The Journal of membrane biology. 154(1)
The conserved leucine residues at the 9' positions in the M2 segments of alpha1 (L264) and beta1 (L259) subunits of the human GABAA receptor were replaced with threonine. Normal or mutant alpha1 subunits were co-expressed with normal or mutant beta1
Publikováno v:
The Journal of membrane biology. 148(2)
alpha 1 and beta 1 subunits of human GABA A receptors were expressed in Sf9 cells using the Sf9-baculovirus system. Better expression was obtained by manipulating the system. Cell growth phase at the time of infection determined the practical range o
Autor:
L. S. Premkumar, P. W. Gage
Publikováno v:
Journal of neurophysiology. 71(6)
1. Single-channel currents were recorded in cell-attached patches on cultured hippocampal neurons in response to gamma-aminobutyric acid-B (GABAB) agonists or serotonin applied to the cell surface outside the patch area. 2. The channels activated by
Publikováno v:
Biochemical Society Transactions. 28:A461-A461