Zobrazeno 1 - 10
of 37
pro vyhledávání: '"P V Sulakhe"'
Publikováno v:
Molecular and cellular biochemistry. 176(1-2)
Chronic exposure of cells to cognate agonists has been established to cause homologous desensitization of G protein-coupled receptors. In this work, we show that exposure of adult rat cardiomyocytes to isoproterenol (ISO) for 24 h led to the desensit
Publikováno v:
Molecular and cellular biochemistry. 175(1-2)
Myocytes were isolated from rat heart ventricles and then incubated with [32P]-sodium phosphate to label intracellular ATP stores. Incubations of the [32P]-labelled cardiomyocytes with a beta-adrenoceptor agonist isoproterenol (10 microM) and with a
Publikováno v:
Molecular and cellular biochemistry. 175(1-2)
The phosphorylation of rat cardiac microsomal proteins was investigated with special attention to the effects of okadaic acid (an inhibitor of protein phosphatases), inhibitor 2 of protein phosphatase 1 and inhibitor of cyclic AMP-dependent protein k
Publikováno v:
Canadian journal of physiology and pharmacology. 74(3)
The main objective of this study was to characterize the alpha 1-adrenoceptors expressed in adult rat brown adipocytes. For this purpose, membrane fractions were prepared from brown adipose tissue as well as from isolated brown adipocytes. The follow
Autor:
S J, Sulakhe, P V, Sulakhe
Publikováno v:
Recent advances in studies on cardiac structure and metabolism. 11
Kinetic properties of guanylate cyclase present in the washed particles, plasma membranes, and the soluble cytoplasm of heart and skeletal muscle are described; properties of the enzyme solubilized by Triton X-100 treatment of the particles or membra
Publikováno v:
FEBS letters. 196(1)
Calcineurin was dissociated into subunits A and B by 6 M urea in the presence (method A) and absence (method B) of MnCl2 and dissociated subunits were isolated by gel filtration in urea in the absence (method B) or presence (method A) of MnCl2. Phosp
Behaviour of cardiac microsomal Ca2+ pump under conditions that may simulate pathological situations
Publikováno v:
General physiology and biophysics. 4(1)
The behaviour of Ca2+ ATPase activity in relation to Ca2+ transport process was studied under different experimental conditions in canine cardiac microsomal fraction predominantly containing sarcoplasmic reticulum. The total Ca2+ concentration requir
Publikováno v:
Recent advances in studies on cardiac structure and metabolism. 11
The binding of [3H]atropine by the primary subcellular fractions and plasma membrane-enriched fractions from atria and ventricles of various species was measured by the Millipore filtration technique. Although all of the primary particulate fractions
Autor:
P J, St Louis, P V, Sulakhe
Publikováno v:
Recent advances in studies on cardiac structure and metabolism. 11
Sarcolemma was isolated by fractionation of salt-extracted particles on two consecutive sucrose density gradients. Salt extraction of homogenates, rather than of washed particles, was found to preserve the activities of adenylate cyclase and ouabain-
Publikováno v:
General physiology and biophysics. 2(5)
The characteristics of Ca2+ release in relation to Ca2+ binding were studied in sarcoplasmic reticulum vesicles isolated from canine myocardium. The Ca2+ binding appeared to be dependent on ATP as a 4 fold increase in Ca2+ binding was observed upon t