Zobrazeno 1 - 10
of 15
pro vyhledávání: '"P T, van Suylichem"'
Publikováno v:
Cell Transplantation, Vol 6 (1997)
To understand why class II Clostridium histolyticum collagenase is much more effective than class I in the isolation of rat pancreatic islets, we analyzed the role of these collagenases in pancreatic tissue dissociation. Crude collagenase was purifie
Publikováno v:
Diabetes. 44:565-571
Publikováno v:
Transplantation proceedings. 27(6)
Autor:
P R, Johnson, P T, Van Suylichem, D L, Roberts, G H, Vos-Scheperkeuter, S A, White, R, Van Schilfgaarde, N J, London
Publikováno v:
Transplantation proceedings. 27(6)
Publikováno v:
Cell Transplantation, Vol 4 (1995)
Because collagen is the major target in the enzymatic dissociation of the pancreas for islet isolation, we determined the amount of collagen and its distribution in a comparative study comprising normal pancreata of rat, dog, man, young pig, and adul
Publikováno v:
Transplantation. 57(7)
Islet isograft function was analyzed after transplantation of 4 well-defined endocrine volumes (12.5%, 25%, 50%, and 100% of the endocrine volume in the normal adult rat pancreas) to 3 different sites (kidney, liver, and spleen). Graft function was t
Publikováno v:
Transplantation proceedings. 26(2)
Publikováno v:
Transplantation proceedings. 26(2)
Publikováno v:
Diabetes research (Edinburgh, Scotland). 19(2)
Rat pancreatic islet yields obtained with dextran gradient purification were compared after suspending the digest into either the top or the bottom layer of the gradient. A 5-layer discontinuous gradient was used, which consisted of 16 ml 31% dextran
Publikováno v:
Hormone and metabolic research. Supplement series. 25
Determination of collagenase and proteolytic activities and slabgel electrophoresis of collagenase preparations obtained from different suppliers reveals a highly variable content of collagenase, proteolytic activities and other components. The elect