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Compartmentalization of mammalian proteins produced in Escherichia coli

Autor: P O Olins, Kristin A. Hogquist, T A Rosenwasser, S Bradford-Goldberg, Jeffrey I. Gordon, David D. Chaplin, S F Nothwehr

Publikováno v: Journal of Biological Chemistry. 265:13066-13073

We have examined the patterns of compartmentalization of several mammalian proteins in Escherichia coli which do not have signal peptides or functional signal peptide equivalents. These proteins include (i) human proapolipoprotein A-I (proapoA-I), a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1070bfa732cfbddc103f09b72f7984ab
https://doi.org/10.1016/s0021-9258(19)38268-7

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Utilization of multiple phage display libraries for the identification of dissimilar peptide motifs that bind to a B7-1 monoclonal antibody

Autor: C. S. Devine, P. A. De Ciechi, M. H. Caparon, P. O. Olins, S. C. Howard, Stephen C. Lee

Publikováno v: Molecular diversity. 1(2)

Seven random peptide libraries (two displaying linear peptides and five displaying cysteine-constrained peptides) were constructed as gene III fusion proteins of the bacteriophage fd-tet. These libraries were used to screen a blocking monoclonal anti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f04be4796312a547235af8c7d193f95
https://pubmed.ncbi.nlm.nih.gov/9237196

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Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli

Autor: G F Barry, Verne A. Luckow, Stephen C. Lee, P O Olins

Publikováno v: Journal of virology. 67(8)

The construction and purification of recombinant baculovirus vectors for the expression of foreign genes in insect cells by standard transfection and plaque assay methods can take as long as 4 to 6 weeks. This period can be reduced to several days by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb42900db54ece55044147e8eaba84b3
https://pubmed.ncbi.nlm.nih.gov/8392598

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Involvement of cysteine residues in catalysis and inhibition of human aldose reductase. Site-directed mutagenesis of Cys-80, -298, and -303

Autor: J M, Petrash, T M, Harter, C S, Devine, P O, Olins, A, Bhatnagar, S, Liu, S K, Srivastava

Publikováno v: The Journal of biological chemistry. 267(34)

In order to study the potential role of cysteinyl residues in catalysis and inhibition of human aldose reductase, mutants containing cysteine to serine substitution at positions 80 (ALR2:C80S), 298 (ALR2:C298S), and 303 (ALR2:C303S) were constructed.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b7995c3605ddca0e84dccfce971bc47e
https://pubmed.ncbi.nlm.nih.gov/1332968

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Compartmentalization of mammalian proteins produced in Escherichia coli

Autor: T A, Rosenwasser, K A, Hogquist, S F, Nothwehr, S, Bradford-Goldberg, P O, Olins, D D, Chaplin, J I, Gordon

Publikováno v: The Journal of biological chemistry. 265(22)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2eacb657637cc7b8eb769e6d66044866
https://pubmed.ncbi.nlm.nih.gov/2115888

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Vector for enhanced translation of foreign genes in Escherichia coli

Autor: P O, Olins, S H, Rangwala

Publikováno v: Methods in enzymology. 185

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cdd068a3051da8e9e3a119ee158eb048
https://pubmed.ncbi.nlm.nih.gov/2199773

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Heparin interferes with the biological effectiveness of atriopeptin

Autor: S W Holmberg, C S Devine, Y F Wei, Philip Needleman, K M Leahy, P O Olins

Publikováno v: Hypertension. 9:607-610

The chromatographic mobility of atriopeptin-28 or of the prohormone is markedly altered by preincubation of the peptides with heparin before separation on reverse-phase high performance liquid chromatography. Protamine prevented the heparin effect an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e50966e19b28d8bed0254e1f28e87530
https://doi.org/10.1161/01.hyp.9.6.607

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Proteolytic processing of atriopeptin prohormone

Autor: M L, Michener, J K, Gierse, R, Seetharam, K F, Fok, P O, Olins, M S, Mai, P, Needleman

Publikováno v: Molecular pharmacology. 30(6)

The metabolism of atriopeptin prohormone ANF1-126 was examined with the aid of two separate radioimmunoassays, one detecting the C-terminal atriopeptins and the other detecting a fragment of the prohormone N-terminus. Intact prohormone standards are

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9944d39788e102d28585c858dd3fe3ac
https://pubmed.ncbi.nlm.nih.gov/2946928

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A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli

Autor: P O, Olins, S H, Rangwala

Publikováno v: The Journal of biological chemistry. 264(29)

We recently reported that a ribosome binding site (RBS) derived from gene 10 of bacteriophage T7 (g10-L) causes a pronounced stimulation of expression when placed upstream of a variety of genes, and that this effect is probably due to a stimulation o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::56047e1ef0fc7145e16b9fde1dbbeb3b
https://pubmed.ncbi.nlm.nih.gov/2676996

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