Zobrazeno 1 - 10
of 14
pro vyhledávání: '"P Joseph Aruscavage"'
Autor:
Claudia D Consalvo, Adedeji M Aderounmu, Helen M Donelick, P Joseph Aruscavage, Debra M Eckert, Peter S Shen, Brenda L Bass
Publikováno v:
eLife, Vol 13 (2024)
Invertebrates use the endoribonuclease Dicer to cleave viral dsRNA during antiviral defense, while vertebrates use RIG-I-like Receptors (RLRs), which bind viral dsRNA to trigger an interferon response. While some invertebrate Dicers act alone during
Externí odkaz:
https://doaj.org/article/a4b7fc7d9f3141feb17511bbe5b926d0
Publikováno v:
eLife, Vol 12 (2023)
Antiviral defense in ecdysozoan invertebrates requires Dicer with a helicase domain capable of ATP hydrolysis. But despite well-conserved ATPase motifs, human Dicer is incapable of ATP hydrolysis, consistent with a muted role in antiviral defense. To
Externí odkaz:
https://doaj.org/article/b8b7ef5a2534413491d9bf54bff6ef46
Publikováno v:
PLoS ONE, Vol 5, Iss 6, p e11217 (2010)
While studying small noncoding RNA in C. elegans, we discovered that protocols used for isolation of RNA are contaminated with small DNA pieces. After electrophoresis on a denaturing gel, the DNA fragments appear as a ladder of bands, approximately 1
Externí odkaz:
https://doaj.org/article/52c50525053e47128cfcb53a93f7595d
Publikováno v:
PLoS ONE, Vol 3, Iss 12, p e4052 (2008)
C. elegans Dicer requires an accessory double-stranded RNA binding protein, RDE-4, to enact the first step of RNA interference, the cleavage of dsRNA to produce siRNA. While RDE-4 is typically essential for RNAi, we report that in the presence of hig
Externí odkaz:
https://doaj.org/article/0606cd392dd5475499f7ff2330d8ae19
Autor:
Loïc Talide, Emilie Lauret, Brenda L. Bass, Carine Meignin, Helen M. Donelick, Matthieu Bellet, Eric R.G.R. Aguiar, João Trindade Marques, P. Joseph Aruscavage
Publikováno v:
RNA
RNA, Cold Spring Harbor Laboratory Press, 2020, 26 (12), pp.1847-1861. ⟨10.1261/rna.077289.120⟩
RNA, Cold Spring Harbor Laboratory Press, 2020, 26 (12), pp.1847-1861. ⟨10.1261/rna.077289.120⟩
In vitro, Drosophila melanogaster Dicer-2 (Dcr-2) uses its helicase domain to initiate processing of dsRNA with blunt (BLT) termini, and its Platform•PAZ domain to initiate processing of dsRNA with 3′ overhangs (ovrs). To understand the relations
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f574475ce14e75f56a1470c3668ba9c4
https://hal.archives-ouvertes.fr/hal-03491818
https://hal.archives-ouvertes.fr/hal-03491818
Autor:
Helen M, Donelick, Loïc, Talide, Matthieu, Bellet, P Joseph, Aruscavage, Emilie, Lauret, Eric R G R, Aguiar, Joao T, Marques, Carine, Meignin, Brenda L, Bass
Publikováno v:
RNA
In vitro, Drosophila melanogaster Dicer-2 (Dcr-2) uses its helicase domain to initiate processing of dsRNA with blunt (BLT) termini, and its Platform•PAZ domain to initiate processing of dsRNA with 3′ overhangs (ovrs). To understand the relations
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bb6180abbef43ff4838dfb21c818087f
https://pubmed.ncbi.nlm.nih.gov/32843367
https://pubmed.ncbi.nlm.nih.gov/32843367
Publikováno v:
Molecular Cell. 58:406-417
In previous studies we observed that the helicase domain of Drosophila Dicer-2 (dmDcr-2) governs substrate recognition and cleavage efficiency, and that dsRNA termini are key to this discrimination. We now provide a mechanistic basis for these observ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de2d43fb780b56074c04764c4ce29cbb
https://doi.org/10.1016/j.molcel.2015.03.012
https://doi.org/10.1016/j.molcel.2015.03.012
Autor:
Noah C. Welker, Ammie A. Krauchuk, Qinghua Liu, Brenda L. Bass, Tuhin S. Maity, Xuecheng Ye, P. Joseph Aruscavage
Publikováno v:
Molecular Cell. 41(5):589-599
The role of Dicer's helicase domain is enigmatic, but in vivo it is required for processing certain endogenous siRNA, but not miRNA. By using Caenorhabditis elegans extracts or purified Drosophila Dicer-2 we compared activities of wild-type enzymes a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::208f40acf0fdf183c0eaab64a9c95b7b
Autor:
Brenda L. Bass, W. Evan Johnson, David A. Nix, Joseph M. Whipple, P. Joseph Aruscavage, Osama A. Youssef, Changjin Hong
Publikováno v:
RNA (New York, N.Y.). 21(5)
Recent studies hint that endogenous dsRNA plays an unexpected role in cellular signaling. However, a complete understanding of endogenous dsRNA signaling is hindered by an incomplete annotation of dsRNA-producing genes. To identify dsRNAs expressed i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92dcb3e29e4b73301e202ed785c05c0d
https://pubmed.ncbi.nlm.nih.gov/25805852
https://pubmed.ncbi.nlm.nih.gov/25805852