A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

Autor: Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L Bain, Marina M Bakhtina, Donald F Becker, Gregory J Bedwell, Ahmet Bekdemir, Tabot M D Besong, Catherine Birck, Chad A Brautigam, William Brennerman, Olwyn Byron, Agnieszka Bzowska, Jonathan B Chaires, Catherine T Chaton, Helmut Cölfen, Keith D Connaghan, Kimberly A Crowley, Ute Curth, Tina Daviter, William L Dean, Ana I Díez, Christine Ebel, Debra M Eckert, Leslie E Eisele, Edward Eisenstein, Patrick England, Carlos Escalante, Jeffrey A Fagan, Robert Fairman, Ron M Finn, Wolfgang Fischle, José García de la Torre, Jayesh Gor, Henning Gustafsson, Damien Hall, Stephen E Harding, José G Hernández Cifre, Andrew B Herr, Elizabeth E Howell, Richard S Isaac, Shu-Chuan Jao, Davis Jose, Soon-Jong Kim, Bashkim Kokona, Jack A Kornblatt, Dalibor Kosek, Elena Krayukhina, Daniel Krzizike, Eric A Kusznir, Hyewon Kwon, Adam Larson, Thomas M Laue, Aline Le Roy, Andrew P Leech, Hauke Lilie, Karolin Luger, Juan R Luque-Ortega, Jia Ma, Carrie A May, Ernest L Maynard, Anna Modrak-Wojcik, Yee-Foong Mok, Norbert Mücke, Luitgard Nagel-Steger, Geeta J Narlikar, Masanori Noda, Amanda Nourse, Tomas Obsil, Chad K Park, Jin-Ku Park, Peter D Pawelek, Erby E Perdue, Stephen J Perkins, Matthew A Perugini, Craig L Peterson, Martin G Peverelli, Grzegorz Piszczek, Gali Prag, Peter E Prevelige, Bertrand D E Raynal, Lenka Rezabkova, Klaus Richter, Alison E Ringel, Rose Rosenberg, Arthur J Rowe, Arne C Rufer, David J Scott, Javier G Seravalli, Alexandra S Solovyova, Renjie Song, David Staunton, Caitlin Stoddard, Katherine Stott, Holger M Strauss, Werner W Streicher, John P Sumida, Sarah G Swygert, Roman H Szczepanowski, Ingrid Tessmer, Ronald T Toth, Ashutosh Tripathy, Susumu Uchiyama, Stephan F W Uebel, Satoru Unzai, Anna Vitlin Gruber, Peter H von Hippel, Christine Wandrey, Szu-Huan Wang, Steven E Weitzel, Beata Wielgus-Kutrowska, Cynthia Wolberger, Martin Wolff, Edward Wright, Yu-Sung Wu, Jacinta M Wubben, Peter Schuck

Publikováno v: PLoS ONE, Vol 10, Iss 5, p e0126420 (2015)

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose o

Externí odkaz: https://doaj.org/article/3bd91e8f42734f39965350067490e003

Rho-dependent Termination within the trp t‘ Terminator. I. Effects of Rho Loading and Template Sequence

Autor: A Q Zhu, P H von Hippel

Publikováno v: Biochemistry. 37:11202-11214

About one-half of the terminators of the Escherichia coli genome require transcription termination factor rho to function. Here we use the very "diffuse" trp t' terminator of E. coli to show that both template sequence and transcript secondary struct

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ce4f20004d909a2af7992dd4c6b7198
https://doi.org/10.1021/bi9729110

Kinetics of the RNA−DNA Helicase Activity of Escherichia coli Transcription Termination Factor Rho. 1. Characterization and Analysis of the Reaction

Autor: Jody M. Dozono, K. M. Walstrom, P H von Hippel, S. Robic

Publikováno v: Biochemistry. 36:7980-7992

The kinetics of the ATP-dependent RNA-DNA helicase activity of Escherichia colitranscription termination factor rho have been analyzed. Helicase substrates were assembled using 255 nt and 391 nt RNA sequences from the trp t' RNA transcript of E. coli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fbe4fce6c43bb9dfc33b18faefee786
https://doi.org/10.1021/bi963179s

Kinetic Parameters of the Translocation of Bacteriophage T4 Gene 41 Protein Helicase on Single-stranded DNA

Autor: Dawn Ring, Mark Young, D. E. Schultz, P H von Hippel

Publikováno v: Journal of Molecular Biology. 235:1447-1458

The bacteriophage T4 gene 41 helicase protein (gp41) carries a single-stranded DNA-dependent ATPase activity that is essential to its helicase activity. This ATPase activity can be stimulated by a wide variety of single-stranded DNA cofactors, includ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::716bfb4cc77e7ae8d397cc0caa77cd06
https://doi.org/10.1006/jmbi.1994.1100

A physical model for the translocation and helicase activities of Escherichia coli transcription termination protein Rho

Autor: S E Seifried, Johannes Geiselmann, Yan Wang, P H von Hippel

Publikováno v: Proceedings of the National Academy of Sciences, Vol. 90, No 16 (1993) pp. 7754-7758

Transcription termination protein Rho of Escherichia coli interacts with newly synthesized RNA chains and brings about their release from elongation complexes paused at specific Rho-dependent termination sites. Rho is thought to accomplish this by bi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5717d40dd2e79218e114dc86c13df719
https://doi.org/10.1073/pnas.90.16.7754