Zobrazeno 1 - 10
of 70
pro vyhledávání: '"P Froussard"'
Autor:
Yunxu Cao, P. Froussard, Hélène Keller, François Xavier Mahon, G. Marit, Josy Reiffers, Jean Ripoche, Maryse Dupouy, Francis Lacombe, Marika Pla, Jacques Thèze, Abdel Elandaloussi
Publikováno v:
European Journal of Haematology. 64:22-31
We investigated transfection rates of CD34+ haematopoietic progenitor cells (HPC) or haematopoietic cell lines (TF-1, KG1a and K562) using the LacZ gene as a reporter and cationic liposomes. The transfection efficiency of CD34+ haematopoietic progeni
Publikováno v:
Gene Therapy. 6:931-938
In contrast to adherent cells, cells growing in suspension and particularly hematopoietic cells, are notoriously difficult to transfect in vitro using nonviral approaches. In the present study, the effect of cell adhesion on gene transfer efficacy wa
IL-2 Receptor α-Chain Expression Is Independently Regulated in Primary and Secondary Lymphoid Organs
Autor:
Christophe Demaison, Laurence Fiette, Valérie Blanchetière, Anneliese Schimpl, Jacques Thèze, P. Froussard
Publikováno v:
The Journal of Immunology. 161:1977-1982
The IL-2R is composed of three chains: IL-2Rα, IL-2Rβ, and IL-2Rγ. In mice, IL-2Rα is critical and determines IL-2 binding to the tripartite IL-2R complex. To extend our previous studies, which demonstrated that IL-2 regulates IL-2Rα expression
Autor:
P Froussard
Publikováno v:
Multiple Sclerosis Journal. 1:88-94
A putative retrovirus called LM7 was recently isolated from a patient with MS. This retrovirus was detected in LM7 and LM711 cultured human leptomeningeal cells. In the present work, nucleic adds from LM711 cell culture supernatants were purified and
Autor:
P. Froussard
Publikováno v:
Genome Research. 2:185-190
T h e polymerase chain reaction (PCR), at first used to amplify known DNA sequences present at very low levels in genomic DNA samples, ~1-3) was rapidly extended to amplif icat ion of RNA sequences by the use of cDNA. ~4's) The PCR method, which requ
Publikováno v:
The Journal of Immunology. 147:2970-2977
Using a cell-free translation system we have expressed the Mr 55,000 subunit of the murine IL-2R (p55 IL-2R), which binds IL-2 with low affinity (Kd = 10 nM). Mutants and truncated forms of p55 IL-2R have been used to map the epitopes recognized by t
Autor:
G, Marit, Y, Cao, P, Froussard, J, Ripoche, M, Dupouy, A, Elandaloussi, F, Lacombe, F X, Mahon, H, Keller, M, Pla, J, Reiffers, J, Theze
Publikováno v:
European journal of haematology. 64(1)
We investigated transfection rates of CD34+ haematopoietic progenitor cells (HPC) or haematopoietic cell lines (TF-1, KG1a and K562) using the LacZ gene as a reporter and cationic liposomes. The transfection efficiency of CD34+ haematopoietic progeni
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 161(4)
The IL-2R is composed of three chains: IL-2R alpha, IL-2R beta, and IL-2R gamma. In mice, IL-2Ra is critical and determines IL-2 binding to the tripartite IL-2R complex. To extend our previous studies, which demonstrated that IL-2 regulates IL-2R alp
Autor:
P. Froussard
Publikováno v:
Nucleic acids research. 20(11)
Publikováno v:
Molecular immunology. 28(1-2)
Two murine T cell lines (C30.1 and Line 1) were used to study the expression of the p55 interleukin-2 receptor gene. C30.1 is an IL-2-dependent T cell line that can be stimulated for a short period of time by IL-4. Line 1 cells are propagated in IL-4