Zobrazeno 1 - 10
of 21
pro vyhledávání: '"P D, Zamore"'
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102336- (2023)
Summary: Binding affinity quantitatively describes the strength of a molecular interaction and is reported by the equilibrium dissociation constant (KD). Here, we present a protocol to measure KD of mammalian microRNA-loaded Argonaute2 protein by dou
Externí odkaz:
https://doaj.org/article/6d1f7c2a85a04687ac738e1d29345a05
Autor:
Tianxiong Yu, Kaili Fan, Deniz M. Özata, Gen Zhang, Yu Fu, William E. Theurkauf, Phillip D. Zamore, Zhiping Weng
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
The pachytene piRNA loci are transcribed by RNA polymerase II in the male germline of placental mammals. Here the authors show that a long first exon or a long unspliced transcript correlates with germline-specific production of piRNA precursor trans
Externí odkaz:
https://doaj.org/article/1760126de9e147de89acee694ee12b89
Autor:
Carlas S. Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, David Grunwald
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
Analysis of single-molecule binding assays still requires substantial manual user intervention. Here, the authors present a pipeline for rapid, automated analysis of co-localization single-molecule spectroscopy images, with a modular user interface t
Externí odkaz:
https://doaj.org/article/843e839540ba4dd28ca6c75bbc68d526
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-14 (2018)
Abstract Background RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every mol
Externí odkaz:
https://doaj.org/article/98cfcae444e64eac8d2cedd188d8344a
Autor:
Yi Lu, Phillip W.L. Tai, Jianzhong Ai, Dominic J. Gessler, Qin Su, Xieyi Yao, Qiang Zheng, Phillip D. Zamore, Xun Xu, Guangping Gao
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 10, Iss , Pp 349-360 (2018)
Corneal neovascularization (NV) is the major sight-threatening pathology caused by angiogenic stimuli. Current drugs that directly target pro-angiogenic factors to inhibit or reverse the disease require multiple rounds of administration and have limi
Externí odkaz:
https://doaj.org/article/c477d019d31e4780b69ad117ac5bd918
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
Abstract Eubacterial ribosomal large-subunit methyltransferase H (RlmH) methylates 23S ribosomal RNA pseudouridine 1915 (Ψ1915), which lies near the ribosomal decoding center. The smallest member of the SPOUT superfamily of methyltransferases, RlmH
Externí odkaz:
https://doaj.org/article/d054ded6d21e412c86951938f4ea5fff
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 6, Iss 10, Pp 3197-3206 (2016)
Adoption of a streamlined version of the bacterial clustered regular interspersed short palindromic repeat (CRISPR)/Cas9 defense system has accelerated targeted genome engineering. The Streptococcus pyogenes Cas9 protein, directed by a simplified, CR
Externí odkaz:
https://doaj.org/article/ca144083ff2a422cb941669ca1370127
Autor:
Yu Fu, Yujing Yang, Han Zhang, Gwen Farley, Junling Wang, Kaycee A Quarles, Zhiping Weng, Phillip D Zamore
Publikováno v:
eLife, Vol 7 (2018)
We report a draft assembly of the genome of Hi5 cells from the lepidopteran insect pest, Trichoplusia ni, assigning 90.6% of bases to one of 28 chromosomes and predicting 14,037 protein-coding genes. Chemoreception and detoxification gene families re
Externí odkaz:
https://doaj.org/article/ace83d72deb04817b8e59d1186487194
Publikováno v:
eLife, Vol 4 (2015)
Many RNAs, including pre-mRNAs and long non-coding RNAs, can be thousands of nucleotides long and undergo complex post-transcriptional processing. Multiple sites of alternative splicing within a single gene exponentially increase the number of possib
Externí odkaz:
https://doaj.org/article/5d826ee328fb40e89f4fc4c7cd27f361
Autor:
Gregory J Babcock, Sowmya Iyer, Heidi L Smith, Yang Wang, Kirk Rowley, Donna M Ambrosino, Phillip D Zamore, Brian G Pierce, Deborah C Molrine, Zhiping Weng
Publikováno v:
PLoS ONE, Vol 9, Iss 6, p e100325 (2014)
Chronic hepatitis C virus (HCV) infection is the most common cause of end-stage liver disease, often leading to liver transplantation, in which case circulating virions typically infect the transplanted liver within hours and viral concentrations can
Externí odkaz:
https://doaj.org/article/443c65ec108248369f81119f193b6e34