Step-by-step full factorial design to optimize a quantitative sandwich ELISA.

Autor: Hernández CA; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Pérez-Bernal M; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba. Electronic address: maylin.perez@cigb.edu.cu., Abreu D; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Valdivia O; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Delgado M; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Dorta D; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Domínguez AG; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Pérez ER; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Sánchez-Ríos JM; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba.

Publikováno v: Analytical biochemistry [Anal Biochem] 2023 Aug 01; Vol. 674, pp. 115195. Date of Electronic Publication: 2023 Jun 01.

Monoclonal antibody generated against Nile tilapia ( Oreochromis niloticus ) IgT heavy chain using a peptide-based strategy.

Autor: Velázquez J; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Cruz L; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Pérez-Bernal M; Research and Development Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Valdivia O; Research and Development Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Haidar A; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Rodríguez A; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Herrera F; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., González O; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Morales A; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Ulloa L; Research and Development Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Blanco R; Production Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Pérez J; Production Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Dorta D; Production Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Luna Y; Production Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Garay HE; Peptides Synthesis Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Abreu DD; Peptides Synthesis Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Ramos Y; Proteomics Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana, 10600, Cuba., Besada V; Proteomics Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana, 10600, Cuba., Cabrera Y; Research and Development Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 83, Sancti Spíritus 60200, Cuba., Estrada MP; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba., Carpio Y; Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), P.O. Box 6162, Havana 10600, Cuba.

Publikováno v: Fish and shellfish immunology reports [Fish Shellfish Immunol Rep] 2023 Apr 14; Vol. 4, pp. 100093. Date of Electronic Publication: 2023 Apr 14 (Print Publication: 2023).

SARS-CoV-2 spike RBD-specific IgA and IgG antibodies in breast milk after vaccination with the protein subunit vaccine Abdala.

Autor: Pérez-Bernal M; Research & Development Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Hernández C; Research & Development Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Ibargollín R; Research & Development Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Martínez M; Neonatology Service, General Hospital 'Camilo Cienfuegos', 128 Bartolome Maso, Sancti Spiritus, Cuba., Soria M; Neonatology Service, General Hospital 'Camilo Cienfuegos', 128 Bartolome Maso, Sancti Spiritus, Cuba., Delgado M; Research & Development Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Valdivia O; Research & Development Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Dorta D; Production Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Domínguez A; Production Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Pérez E; Research & Development Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba., Cabrera Y; Research & Development Department, Center for Genetic Engineering and Biotechnology, Circunvalante Norte, Olivos III, Sancti Spiritus, Cuba.

Publikováno v: Infectious medicine [Infect Med (Beijing)] 2022 Dec; Vol. 1 (4), pp. 253-261. Date of Electronic Publication: 2022 Nov 18.

Murine monoclonal antibodies against RBD of the SARS-CoV-2 spike protein as useful analytical tools for subunit vaccine development and clinical trials.

Autor: Blanco OR; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Dorta D; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Hernández CA; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Abreu D; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Domínguez AG; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Luna Y; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Valdivia O; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Pérez-Bernal M; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Tamayo C; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Lemos G; Center for Genetic Engineering and Biotechnology, Ave. 31 e/ 158 y 190, Playa, Havana, Cuba., Pasarón IM; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Pérez JJ; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Benítez L; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba., Bequet-Romero M; Center for Genetic Engineering and Biotechnology, Ave. 31 e/ 158 y 190, Playa, Havana, Cuba., Fragas A; Civilian Defense Scientific Research Center, Carretera de Jamaica y Autopista 15 Nacional, San José de las Lajas, Mayabeque, Cuba., Cabrera Y; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba. Electronic address: yeosvany.cabrera@cigb.edu.cu., Pérez ER; Center for Genetic Engineering and Biotechnology of Sancti Spíritus, Circunvalante Norte, Olivos III, Sancti Spíritus, Cuba.

Publikováno v: Journal of immunological methods [J Immunol Methods] 2022 Jan; Vol. 500, pp. 113195. Date of Electronic Publication: 2021 Nov 26.

Marker-free Transgenic Rice Lines with a Defensin Gene are Potentially Active against Phytopathogenic Fungus Sarocladium oryzae.

Autor: PÉREZ-BERNAL, M., DELGADO, M., CRUZ, A., ABREU, D., VALDIVIA, O., ARMAS, R.

Publikováno v: Acta Phytopathologica et Entomologica Hungarica; Dec2017, Vol. 52 Issue 2, p135-144, 10p