Zobrazeno 1 - 10
of 88
pro vyhledávání: '"Pål Nyrén"'
Publikováno v:
BioTechniques, Vol 26, Iss 4, Pp 728-734 (1999)
A real-time, simple and sensitive method for detection of nucleoside diphosphate (NDP) kinase activity has been developed. The assay is based on detection of ATP, generated in the NDP kinase reaction between a nucleoside triphosphate and adenosine di
Externí odkaz:
https://doaj.org/article/218f38c93a474786ab8733ea4e905a05
Publikováno v:
BioTechniques, Vol 25, Iss 5, Pp 876-884 (1998)
The need for a primer hybridization step before sequencing has been eliminated using a stem-loop structure generated by PCR. The loop structure is obtained by careful design of the PCR primer or by cloning the target DNA into a dedicated vector (pRIT
Externí odkaz:
https://doaj.org/article/4ba911282cbe462b8907990e96d97d22
Publikováno v:
BioTechniques, Vol 24, Iss 2, Pp 302-306 (1998)
A simple and sensitive technique for detection of reverse transcriptase (RT) activity in real time has been developed. The technique is based on continuous detection of the inorganic pyrophosphate formed in the RT-catalyzed reaction by a luminometric
Externí odkaz:
https://doaj.org/article/32b53e7cc6c545a58ed865112f65bd21
Autor:
Anders F Andersson, Mathilda Lindberg, Hedvig Jakobsson, Fredrik Bäckhed, Pål Nyrén, Lars Engstrand
Publikováno v:
PLoS ONE, Vol 3, Iss 7, p e2836 (2008)
Humans host complex microbial communities believed to contribute to health maintenance and, when in imbalance, to the development of diseases. Determining the microbial composition in patients and healthy controls may thus provide novel therapeutic t
Externí odkaz:
https://doaj.org/article/59bd4257a8e74e108eb3a089ba783980
Autor:
Michael S Akhras, Magnus Unemo, Sreedevi Thiyagarajan, Pål Nyrén, Ronald W Davis, Andrew Z Fire, Nader Pourmand
Publikováno v:
PLoS ONE, Vol 2, Iss 9, p e915 (2007)
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which e
Externí odkaz:
https://doaj.org/article/5e957c84cac440b58e8f5225d3a312cb
Autor:
Michael S Akhras, Sreedevi Thiyagarajan, Andrea C Villablanca, Ronald W Davis, Pål Nyrén, Nader Pourmand
Publikováno v:
PLoS ONE, Vol 2, Iss 2, p e223 (2007)
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonst
Externí odkaz:
https://doaj.org/article/883728b1dfbb4f60b6c07f784260507f
Publikováno v:
Biomedical Microdevices. 14:625-629
Optimization of targeted cell capture with microfluidic devices continues to be a challenge. On the one hand, microfluidics allow working with microliter volumes of liquids, whereas various applications in the real world require detection of target a
Publikováno v:
Seibutsu Butsuri. 47:129-132
Autor:
Pål, Nyrén
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1315
One late afternoon in the beginning of January 1986, bicycling from the lab over the hill to the small village of Fullbourn, the idea for an alternative DNA sequencing technique came to my mind. The basic concept was to follow the activity of DNA pol
Autor:
Pål Nyrén
Publikováno v:
Methods in Molecular Biology ISBN: 9781493927142
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f74775285d299a125fd4ceabfaf3b11
https://doi.org/10.1007/978-1-4939-2715-9_1
https://doi.org/10.1007/978-1-4939-2715-9_1