Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Owen Becette"'
Publikováno v:
Molecules, Vol 24, Iss 19, p 3476 (2019)
RNA structure and dynamic studies by NMR spectroscopy suffer from chemical shift overlap and line broadening, both of which become worse as RNA size increases. Incorporation of stable isotope labels into RNA has provided several solutions to these li
Externí odkaz:
https://doaj.org/article/b5eebae9be70447e900386e47315a1b4
Publikováno v:
Monatshefte für Chemie - Chemical Monthly. 152:1361-1367
We describe the hybrid enzymatic and chemical synthesis of a 2′-O-cyanoethoxymethyl (CEM) [1′,6-13C2, 5-2H]-uridine phosphoramidite (amidite). This is the first report of an atom-specific nucleobase and ribose labeled CEM amidite. Importantly, th
Publikováno v:
Monatshefte für Chemie - Chemical Monthly. 152:441-447
Solution NMR of the magnetically active fluorine-19 (19F) isotope offers tremendous advantages in the study of RNA structure and conformational changes. However, exploiting these advantages requires the synthesis of fluorinated RNA-building blocks, a
Publikováno v:
Science Advances
19F-13C spy couple enables NMR visualization of long-lived signals of large RNA structures, folds, and drug binding pockets.
RNAs form critical components of biological processes implicated in human diseases, making them attractive for small-mol
RNAs form critical components of biological processes implicated in human diseases, making them attractive for small-mol
Publikováno v:
Journal of biomolecular NMR. 74(6-7)
Many regulatory RNAs undergo dynamic exchanges that are crucial for their biological functions and NMR spectroscopy is a versatile tool for monitoring dynamic motions of biomolecules. Meaningful information on biomolecular dynamics requires an accura
Publikováno v:
Biophysical Journal. 116:356a
Selective Isotope Labeling to Facilitate Structural and Dynamics Studies of RNAs by NMR Spectroscopy
Publikováno v:
Biophysical Journal. 116:356a
Autor:
Owen Becette, Regan M. LeBlanc, Victoria D'Souza, Christoph Kreutz, T. Kwaku Dayie, Christoph H. Wunderlich, Carolina Salguero, Andrew P. Longhini, Bruce A. Johnson
Publikováno v:
Nucleic Acids Research
Stable isotope labeling is central to NMR studies of nucleic acids. Development of methods that incorporate labels at specific atomic positions within each nucleotide promises to expand the size range of RNAs that can be studied by NMR. Using recombi