Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Owen A. Huynh"'
Autor:
Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extrac
Publikováno v:
Biotechnologies for Plant Mutation Breeding ISBN: 9783319450193
Single-strand-specific nucleases are used to cleave mismatches in otherwise double-stranded DNA. Assays typically involve PCR amplification followed by a denaturing and annealing step to generate heteroduplexed DNA molecules from PCR products contain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7143fc62f9b71b7b7ed3ae8e55442e0
https://doi.org/10.1007/978-3-319-45021-6_15
https://doi.org/10.1007/978-3-319-45021-6_15
Publikováno v:
Plant Biotechnology Journal
Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba3f8018b21ed69089fc87a2651b5882
https://doi.org/10.1111/j.1467-7652.2012.00733.x
https://doi.org/10.1111/j.1467-7652.2012.00733.x
Autor:
Mayada M. Beshir, Bradley J. Till, Owen A. Huynh, Joanna Jankowicz-Cieslak, Robert G. Laport, Bernhard J. Hofinger
Publikováno v:
Low-Cost Methods for Molecular Characterization of Mutant Plants ISBN: 9783319162584
PCR is used to amplify regions to be interrogated for the presence of mutations (SNP and small indel polymorphisms). While PCR is a common practice and many protocols exist, reaction conditions are provided here that are optimized for TILLING and Eco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::239865181e3e31044a4b39a970b4fa19
https://doi.org/10.1007/978-3-319-16259-1_5
https://doi.org/10.1007/978-3-319-16259-1_5
Autor:
Bradley J. Till, Robert G. Laport, Mayada M. Beshir, Joanna Jankowicz-Cieslak, Owen A. Huynh, Bernhard J. Hofinger
Publikováno v:
Low-Cost Methods for Molecular Characterization of Mutant Plants ISBN: 9783319162584
A crude celery extract containing the single-strand-specific nuclease CEL I, has been widely used in TILLING and Ecotilling projects around the world. Yet, celery is hard to come by in some countries. Sequences homologous to CEL I can be found in dif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::907c96c187454c89843fe41d62e915a3
https://doi.org/10.1007/978-3-319-16259-1_7
https://doi.org/10.1007/978-3-319-16259-1_7
Autor:
Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
Publikováno v:
Low-Cost Methods for Molecular Characterization of Mutant Plants ISBN: 9783319162584
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4ed9c714c254532a0fd0b4fa8da437b2
https://doi.org/10.1007/978-3-319-16259-1_8
https://doi.org/10.1007/978-3-319-16259-1_8
Autor:
Bernhard J. Hofinger, Robert G. Laport, Owen A. Huynh, Mayada M. Beshir, Joanna Jankowicz-Cieslak, Bradley J. Till
Publikováno v:
Low-Cost Methods for Molecular Characterization of Mutant Plants ISBN: 9783319162584
The methods described in this chapter were developed to avoid toxic organic phase separation utilized in many low-cost DNA extraction protocols such as the CTAB method. The steps involve: (1) lysis of the plant material, (2) binding of DNA to silica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dfc9081fac0fbc4de763d1f6df2b5e0e
https://doi.org/10.1007/978-3-319-16259-1_4
https://doi.org/10.1007/978-3-319-16259-1_4
Autor:
Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
Publikováno v:
Low-Cost Methods for Molecular Characterization of Mutant Plants ISBN: 9783319162584
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d15f0e7f07d7318e995fe5063e27a38e
https://doi.org/10.1007/978-3-319-16259-1_1
https://doi.org/10.1007/978-3-319-16259-1_1
Autor:
Bradley J. Till, Owen A. Huynh, Joanna Jankowicz-Cieslak, Robert G. Laport, Bernhard J. Hofinger, Mayada M. Beshir
Publikováno v:
Low-Cost Methods for Molecular Characterization of Mutant Plants ISBN: 9783319162584
All laboratories should have standardized health and safety rules and practices. These can vary from region to region due to differences in legislation. Before beginning new experiments, please consult your local safety guidelines. Failure to follow
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::53e530f42227653259131b8c5062a3b0
https://doi.org/10.1007/978-3-319-16259-1_2
https://doi.org/10.1007/978-3-319-16259-1_2
Autor:
Robert G. Laport, Joanna Jankowicz-Cieslak, Mayada M. Beshir, Bernhard J. Hofinger, Bradley J. Till, Owen A. Huynh
Publikováno v:
Low-Cost Methods for Molecular Characterization of Mutant Plants
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extract
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2000454649b644fdab6421b1df57a637
https://doi.org/10.1007/978-3-319-16259-1
https://doi.org/10.1007/978-3-319-16259-1