Výsledky vyhledávání - "Ouided Friaa"

Coincidence Measurements in Dual-Color Confocal Microscopy: A Combined Single-Particle and Fluorescence Correlation Approach

Autor: Cécile Fradin, Ouided Friaa

Publikováno v: Biophysical Reviews and Letters. :249-271

In this paper we discuss how the coincident detection of mobile particles in dual-color confocal images can be improved. Optimal coincidence detection requires a careful choice of experimental conditions and image acquisition parameters in order to m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f9bc43000e0548f57259f3ee357aace5
https://doi.org/10.1142/s1793048014400074

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The Proapoptotic Protein tBid Forms Both Superficially Bound and Membrane-Inserted Oligomers

Autor: Nehad Hirmiz, Brian Leber, Ouided Friaa, David W. Andrews, Martin Kurylowicz, Cécile Fradin, Pourya Masoudian, Aisha Shamas-Din, Sanjeevan Shivakumar, Yaseen Manan

Publikováno v: Biophysical Journal. 106(10):2085-2095

Bid is a proapopotic activator protein of the Bcl-2 family that plays a pivotal role in controlling mitochondrial outer membrane permeabilization during apoptosis. Here, we characterized the interaction of fluorescently labeled truncated Bid (tBid) w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c94c8f009b5430b02e9d875150b7142

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Optimizing the Acquisition and Analysis of Confocal Images for Quantitative Single-Mobile-Particle Detection

Autor: Melissa Furukawa, David W. Andrews, Cécile Fradin, Ouided Friaa, Brian Leber, Aisha Shamas-Din

Publikováno v: ChemPhysChem. 14:2476-2490

Quantification of the fluorescence properties of diffusing particles in solution is an invaluable source of information for characterizing the interactions, stoichiometry, or conformation of molecules directly in their native environment. In the case

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2014deb1da1b90601264784c62e8b38a
https://doi.org/10.1002/cphc.201201047

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Characterizing Bax and Bid Binding to Liposomes and Planar Membranes with Single Molecule Resolution

Autor: Ouided Friaa, Cécile Fradin, Sanjee Shivakumar

Publikováno v: Biophysical Journal. 102(3)

The Bcl-2 family proteins regulate the initiation of apoptosis through protein-protein interactions that are often membrane dependant. The proapoptotic protein Bax is an attractive drug target to modulate apoptosis since it plays a vital role in mito

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Detection of Mobile Single Fluorescent Particles by Confocal Microscopy: A Comparison with Fluorescence Correlation Techniques

Autor: Ouided Friaa, Cécile Fradin, Aisha Shamas-Din, Melissa Furukawa, Radhika Voleti, Sanjeevan Shivakumar

Publikováno v: Biophysical Journal. 100(3)

To improve our understanding of proteins interactions with cellular membranes, we have developed a method based on confocal microscopy that enable us to correlate the number of proteins bound to a lipid vesicle with the vesicle properties (membrane i

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Reaction between the anesthetic agent propofol and the free radical DPPH in semiaqueous media: kinetics and characterization of the products

Autor: Vincent Chaleix, Ouided Friaa, Marc Lecouvey, Daniel Brault

Publikováno v: Free Radical Biology and Medicine
Free Radical Biology and Medicine, Elsevier, 2008, 45 (7), pp.1011-1018. ⟨10.1016/j.freeradbiomed.2008.07.001⟩
Free Radical Biology and Medicine, 2008, 45 (7), pp.1011-1018. ⟨10.1016/j.freeradbiomed.2008.07.001⟩

0891-5849; The reaction of the free radical diphenylpicrylhydrazyl (DPPH[dot above]) with the anesthetic agent 2,6-diisopropylphenol (propofol, PPF) was investigated in buffered hydroalcoholic media. The kinetics was followed using a stopped-flow sys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::888463e591169963565199294b3533cc
https://hal.archives-ouvertes.fr/hal-00416617

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Quantification of Protein Distribution on Liposomes using Confocal Microscopy: A Single Mobile Fluorescent Particle Detection Method

Autor: Cécile Fradin, Melissa Furukawa, Brian Leber, Aisha Shamas-Din, David W. Andrews, Ouided Friaa, Radhika Voleti, Sanjeevan Shivakumar

Publikováno v: Biophysical Journal. 102(3):51a-52a

Bcl-2 family proteins play a crucial role in the regulation of the permeabilization of the outer mitochondrial membrane (OMM), which is one of the main steps leading to cell death through apoptosis.To a first approximation, apoptosis is controlled by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32ed58eeb92b6f42ef61b46d6dc0f864

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Kinetics of the reaction between the antioxidant Trolox? and the free radical DPPH? in semi-aqueous solution

Autor: Daniel Brault, Ouided Friaa

Publikováno v: Organic & Biomolecular Chemistry. 4:2417

Reaction of the free-radical diphenylpicrylhydrazyl (DPPH(.)) with Trolox (TrOH) was investigated in buffered hydroalcoholic media by using a stopped-flow system. DPPH was reduced to the hydrazine analogue DPPH-H with a measured stoichiometry of abou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22dde4ea98faf2c3b8e5ce6d39c6079b
https://doi.org/10.1039/b602147f

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